TITLE
De novo generation of casein micelles
SHORT PROJECT DESCRIPTION
1$0($1'326,7,21
Thea Lykkegaard Møller, PhD-student
START/END DATE
9/1/19 8/31/21
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It has not been decided yet due to covid-19 pandemic
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No publications yet.
Planned conferences: NIZO Ingredients conference,
Food Colloids conference at Lund University,
Northern Lights on Food (NLF) conference in Lund
PICTURE RELATED TO THE PROJECT
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Thea Lykkegaard Møller, FOOD, Aarhus University
Milena Corredig, iFOOD, Aarhus University
Jan Skov Pedersen, iNANO, Aarhus University
Arla Foods Ingredients
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(MAX. 200 WORDS IN TOTAL)
In the industry, microfiltration (MF) is one of the most
widespread and effective techniques for separation
of components in liquids. As this membrane process
becomes more widespread, it is important to
understand what are the processing conditions and,
in particular, the chemical and physical modifications
to the milk, that will result in permeate and
retentates of different composition and functionality.
With this PhD project, we seek to understand the
assembly properties of casein proteins with modified
soluble fractions, and the structure of the remaining
colloidal particles.
The overall objective of the PhD project is to
understand if it is possible to create, de novo,
different casein particles by processing.
In the work performed so far we have created casein
dissociation by changing environmental and
chemical conditions such as mineral concentration,
pH, temperature and phosphorylation degree of the
caseins. The micellar phase as well as the soluble
phase were characterized by a combination of
chemical characterization techniques and structural
analyses by Light (LS) and Small Angle X-ray
(SAXS) Scattering. We found that Ca seem to play a
major role in the heat-stability of the micelle and that
structural changes can be observed by SAXS as a
consequence of heat-treatment and
dephosphorylation. A new model describing the
hierarchical structure of the micelle has been
proposed based on the SAXS data.
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Dairy protein chemistry
Physical analysis by SAXS
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Next steps entail the reassembly of isolated casein
fractions to create new particles.
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