Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 71 - December 2022 https://hab.ioc-unesco.org/ The GlobalHAB mini-symposium on automated plankton observations High-frequency observations of Harmful Algal Blooms (HABs) and grazers of HAB species are needed to be able to understand HAB dynamics, to develop predictive models of HABs and to produce robust warnings of HABs. In recent years, novel in situ instrumentation has been developed for automated high-frequency HAB detection in nearreal time. Automated plankton imaging-inflow produces high quality images of phytoplankton larger than 5-10 μm including many harmful algae (Fig.1). There are four main approaches illustrated in Fig. 2: (1) Imaging-in-flow which is based on the morphology of organisms, (2) flow cytometry which is based on fluorescence properties (e.g., pigmentation) and scattering properties (e.g. size) of cells, (3) observations of bulk bio-optical properties (pigment based) of plankton communities, e.g. fluorescence, absorption and turbidity and (4) molecular methods. The minisymposium was focused on the imaging-in-flow systems which give detailed information about HAB organisms, including some instrumentation which provided both imaging-in-flow and cytometric data. In addition, imaging-inflow instruments for observing grazers, i.e., microzooplankton and multicellular zooplankton were included. These instruments are now being adopted in research and piloted in monitoring programmes. The aim of the mini-symposium was to bring together experts on, and users of, automated imaging-inflow systems to present methods and recent results and to share experiences. Another aim was to carry out a comparison of results when analysing plankton communities quantitatively. Young scientists were particularly encouraged to attend the symposium. The minisymposium/workshop on automated plankton observations took place at Content Featured article GlobalHAB mini-symposium on automated plankton observations Bengt Karlson, Elisa Berdalet & Raphael M Kudela.......................... 1 High-Biomass HABs Lepidodinium chlorophorum in the Chilean fjords......................... 5 Benthic HABs Seaweeds of Rangitāhua hide toxic BHABs......................................... 7 Gambierdiscus and other BHABs isolated from Tonga.......... 9 Joint New Zealand Japan project to map and predict BHABs.................................................... 13 Networking, Conferences and International Cooperation The Canadian Citizen Science Oceanography Program Canada................................................... 15 Dino 12 meeting................................ 17 Dino 12 Workshop............................ 18 XIV Iberian Meeting on Harmful Algae and Biotoxins (REDIBAL)............................................ 19 ICES-IOC Working Group on Harmful Algal Bloom Dynamicsmeeting ................................................ 20 Caribbean project CARMINA........ 21 Forthcoming 20th ICHA Conference in Hiroshima............................................. 22 NEW !! 19 ICHA Proceedings....................... 23 Fig. 1. Micrographs of phytoplankton observed during the mini-symposium. Scale is not consistent. A-C. Dinophysis acuta (field sample), A. Light microscope, photo: Mona Ring Kleiven. B. IFCB and C. FlowCam. D-E. Phaeocystis globosa (culture), D. IFCB, E. CytoSense/Cytobuoy and F. FlowCam. G-I. Pyramimonas sp. (culture), G. IFCB, H. Cytosense/Cytobuoy and I. FlowCam Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 71 - December 2022 https://hab.ioc-unesco.org/ The GlobalHAB mini-symposium on automated plankton observations High-frequency observations of Harmful Algal Blooms (HABs) and grazers of HAB species are needed to be able to und low-cost alternative to the commercial instruments. The PlanktoScope can be used with objectives with different magnifications. At present, the PlanktoScope (version 4) is somewhat limited in functionality compared to the commercial alternatives and it may only be applicable to multicellular zooplan Fig 3. Instruments used in the mini-symposium: Three Imaging FlowCytobots (IFCBs) (top left) and two Cytosense/Cytobuoys (top right), with their water proof housing removed during laboratory work, and the FlowCam 8400 (bottom left) and one FlowCam Macro (not shown) were used during the mini-symposiu Fig. 4. The principle for using expert trained AI-machine learning for automated analysis of images acquired using imaging-in-flow instruments Acknowledgements This mini-symposium was sponsored by the SCOR and IOC/UNESCO GlobalHAB program (www.globalhab.info), NOAA, the ICES/IOC WGHABD, the Univers High-Biomass Harmful Algal Blooms (HB-HAB) in the Chilean Fjords System: Lepidodinium chlorophorum Harmful Algal Blooms (HABs) have become a recurrent problem in Southern Chile (Patagonian fjords) during the last decades, responsible for large scale events affecting public health and the aquaculture Fig. 2. Vertical distribution of A) phytoplankton cell counts, and B) temperature (red line), salinity (blue line) and chlorophyll-a (green line). A similar event occurred in the same area during March and April 2003 (cell densities of 13x106 cells L-1 and Chl-a concentrations of ~1000 μg L-1), cau Seaweeds of Rangitāhua hide toxic dinoflagellates Fig. 1. Raoul Island (left), MY Dapple (right). In November 2021 a team of scientists joined the Expedition Rangatahi to Rangitāhua/Kermadec Islands, a New Zealand territory approximately 1000 km north east of New Zealand. This was the first scient Table 1. Dinoflagellate species isolated from samples collected at Denham Bay Observation Site, Raoul Island, Rangitāhua Kermadec Islands, November 2021 Species Macroalga host CICCM code Amphidinium carterae Dictyota aff. pfaffi ns A. massartii Caulerpa racemosa CAWD377 Coolia canariensis First isolation of toxin-producing benthic dinoflagellates from the Kingdom of Tonga, including the ciguatera causing genus Gambierdiscus Fig. 1. Map of the Kingdom of Tonga showing study sites visited in June 2016 and 2017 across three island groups: Haapai, Vavau, and Tongatapu. Sites where live Fig. 3. (left) Phoebe Argyle working in in Fangautu lagoon, Tongatapu and (right) Neiafu Tahi, Vavau. Earlier reports of CP in Tonga indicate the presence of at least one toxin producing Gambierdiscus species, but there is limited information on the geographical distribution. During two comprehensi no Gambierdiscus cells were successfully cultured from these samples even though they were visually present in the samples. In 2017, one Prorocentrum lima and one Coolia tropicalis (Fig. 5e-f) isolate were successfully established into culture (Table 1). The Prorocentrum lima isolate was collected f Table 1. Dinoflagellate isolates successfully established into culture from the Kingdom of Tonga in 2016 and 2017. Details include collection site, macroalgae species the cells were isolated from, species identification of the isolates, toxin production, CICCM identification code, and species identi Aotearoa New Zealand Japan joint research project on mapping the predicted distribution of ciguatera poisoningrelated benthic dinoflagellates after forecasted global warming Aotearoa New Zealand and Japan are maritime nations with strong cultural, recreational, and economic connections to seafood. years by the Royal Society of New Zealand (RSNZ) and the Japan Society for the Promotion of Science (JSPS). The project title is Mapping the predicted distribution of toxic benthic microalgae after forecasted global warming in Japan and New Zealand. The project aims to develop a pipeline for assessi Human Dimensions of the Citizen Science Oceanography Program Operated by the Pacific Salmon Foundation, Canada Citizen Science involves members of the general public taking part in certain scientific work (e.g. data or sample collection), often in collaboration with or under the direction of profess Cooperation scenes from the Citizen Science Oceanography Program, Strait of Georgia, Canada zen science programs is the ongoing involvement of highly motivated volunteers, interested scientists who provide oversight and guidance, and continuing funding. With an apparent increase in intensity and du 12th International Conference on Modern and Fossil Dinoflagellates Fig. 1. Karin Zonneveld paying homage to the I Dino conference in 1978 Forty-four years after the Penrose Conference on Modern and Fossil Dinoflagellates (Fig. 1), the long awaited DINO12 conference was held 4-8 July 2022 at the Au DINO 12 Workshop on Sampling Methods for Benthic Dinoflagellates Unlike planktonic species, there is no standardized, or even agreed upon, quantitative method for sampling benthic dinoflagellates. During the workshop, methods traditionally used for collecting benthic dinoflagellates (BHABs) were rev XIV Iberian Meeting on Harmful Algae and Biotoxins (REDIBAL) The XIV Iberian Meeting on Harmful Algae and Biotoxins was held in Lisbon, Portugal, from 1st 3rd June at the Magalhães Auditorium of the Portuguese Institute of Sea and Atmosphere (IPMA). The event was organized in a joint cooperation be ICES-IOC Working Group on Harmful Agal Bloom Dynamics Meeting The ICES-IOC Working Group on Harmful Algal Bloom Dynamics (WGHABD) is an important forum for ICES (International Council for the Exploration of the Seas) and the IOC of UNESCO to review and discuss HAB events and to provide annual advice CARMINA The CARMINA Project (CARibbean MIcro-algae respoNsible for ciguAtera poisoning: Diversity, Toxicity and Toxin Production) with 20 participants from 11 island nations and mainland Caribbean countries, France and the USA held its virtual kick-off meeting (Fig 1). The countries both in and surr ISSHA's corner Invitation to ICHA 2023 Hiroshima Dear ISSHA members and colleagues, Please save the date! The 20th International Conference on Harmful Algae (ICHA) will be held at the lovely Grand Prince Hotel Hiroshima, Japan from the 5th to the 10th of November 2023. The organizing committee of c NEW ! The Proceedings of the 19th International Conference on Harmful Algae 2021 Mexico have been published. Each of the 47 contributions have been assigned a DOI identifier which can be found on the bottom of the first page of each manuscript. Eight case studies of Early Warning Systems for Harmful