Table 1. Toxin concentration per sample. Toxins screened included PTX1 (pectenotoxin-1), PTX2 (pectenotoxin-2) and YTX (yessotoxin). DA (domoic acid), SPX1 (spirolide-1), GYM (gymnodimine), OA (okadaic acid), DTX1 (dinophysistoxin-1), DTX2 (dinophysistoxin-2), AZA1 (azaspiracid-1), AZA2 (azaspiracid-2), AZA3 (azaspiracid-3), 45-OH-YTX (45-hydroxyYTX), 45-OH-homo-YTX (45-hydroxy-homo-YTX) were analyzed with no detectable amounts recorded. Each result is given in pg per cell. ND refers to not detectable. respectively. Dinoflagellate strains from the bloom samples were also isolated and established in axenic culures. Their 8S ribosomal DNA was analyzed using conventional Sanger sequencing techniques. The toxicity of PTXs, YTXs and analogs to humans has yet to be confirmed however these lipophilic toxins may cause cellular death, apoptosis and other damages in mouse neuroblastoma cell tests. PTXs and DSP (diarrhetic shellfish poisoning) toxins are both produced by Dinophysis species and often co-occur in shellfish samples. The frequent co-occurrence of DSP with PSP (paralytic shellfish poisoning) toxins in Chile, presents additional complications to the application of standard toxin detection methods [10-13]. Previous research on shellfish (bivalves, gastropods) toxins has been carried out mainly in southern Chile. Further studies should be developed, especially on rapid-detection methods, to focus on local seafood products in northern Chile. Little is known about the toxin profiles and toxicity of shellfish in this region.. This lack of information presents a potential threat to seafood exports from Antofagasta and other high production areas in northern Chile [14]. Acknowledgements 2. Kang HE et al 2018. PeerJ6: e4854 3. Tamele IJ et al 2019. Toxins 11(1): 58 4. Visciano P et al 2016. Front Microbiol 7: 1051 5. Silva M et al 2015. Toxins 7(3): 859-885 6. Fujiki H et al 1981 Proc Natl Acad Sci USA 78: 3872-3876 7. Maciel-Baltazar E 2015. Hidrobiológica 25(3): 382-389 8. Lim AS et al 2014. Harmful Algae 37: 53-61 9. Zhou Y et al 2017. Sci Total Environ 574: 499-508 10. Fernández ML 2000. S Afr J mar Sci 22: 339346 11. DellOvo V et al 2008. Toxicol Sci 106: 392-399 12. Toyofuku H 2006. Mar Pollut Bull 52: 1735-1745 13. Cañete E et al 2008. Toxicon 52: 541-550 14. Alarcan J et al 2018. Mar Drugs 16(2): 46 Authors Vladimir Ávalos, Henry Cameron, Stephanie Barría & Carlos Riquelme, Centro de Bioinnovación Antofagasta (CBIA), Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta, Chile We acknowledge the use of images from the NASA Worldview application (https://worldview.earthdata.nasa.gov/), part of the NASA Earth Observing System Data and Information System (EOSDIS) and the financial support from FONDEF Grant IT17F10001 & MACHI SATREPS. Oscar Espinoza & Leonardo Guzmán, Centro de Estudios de Algas Nocivas (CREAN), Instituto de Fomento Pesquero, Puerto Montt, Chile References 1. Zohdi E & M Abbaspour 2019. Int J Environ Sci Technol 16: 1789 Email corresponding author: v.avalos.ovando@gmail.com; carlos.riquelme@uantof.cl Kyoko Yarimizu, Makoto Okazaki & Satoshi Nagai, Research Center for Aquatic Genomics, National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, Yokohama, Kanagawa, Japan Fig. 2. Clear field microscopy image of a sample collected from station PUERTO during the bloom in Antofagasta in December 2018. The image shows a variety of dinoflagellates. All green arrows flag a number of different organisms groups 1. Protoperidinium cf steinii; 2. Prorocentrum sp. 3. Dictyocha; 4. Thecate dinoflagellate (possibly apical view of Peridinium sp..; 5. Tripos furca; 6. Possibly Gambierdiscus sp.; 7. Prorocentrum triestinum, 8. Prorocentrum micans.; 9. Dinophysis acuminata complex. 10. Thecate dinoflagellate (possibly apical view of Alexandrium sp.; 11. Gymnodinium sp.; 12. Entomoneis sp.; 13. Cylindrotheca sp. The yellow arrows indicate two diatoms observed in the sample, and the red arrows indicate the remaining dinoflagellates HARMFUL ALGAE NEWS NO. 62 / 2019 15 Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 62 - June 2019 www.ioc-unesco.org/hab The 18th International Conference for Harmful Algae Twenty five years after the 6th International Conference on Toxic Marine Phytoplankton (6th ICHA, 1993), experts on harmful algal bloom Fig. 2. Ice-breaking at La Cité, Nantes, during the 18th ICHA, 2018 SHA society members were kept very busy, fully engaged with the evaluation of student presentations to choose the candidates for the Maureen Keller awards, the election of Yasumoto lifetime achievement awards, voting for future con Scientific and social scenes from participants during the 18th ICHA, Nantes, 2018. HARMFUL ALGAE NEWS NO. 62 / 2019 3 Scientific Highlights of the 18th Inter national Conference on Harmful Algae Plenary talks and Yasumoto awards Each morning and afternoon session began with plenary speakers who covered a number of topics including: climate change impacts on inland and coastal eutrophication (Anna M. Michalek), chem Maria Immacolata Ferrante investigated the Pseudo-nitzschia multistriata sexual cycle and found the gene that determines the mating type in this pennate diatom. Pseudo-nitzschia is found globally in the Tara Oceans dataset and the genes expressed during the sexual phase can track this important but Fig. 2. Extracellular vesicles in Alexandrium minutum. From left to right: Vegetative cell under light microscopy; epifluorescence microscopy showing the cell chloroplasts in red; vesicles in green due to lipid stain PKH67, and composite of the epifluorescence images, theca of the cell is in blue, Fig. 3. Co-evolutionary arms race. Diatoms sense presence of copepods via presence of chemical cues (copepodamides), and respond by producing domoic acid which then affect the copepods (reduced escape response). Photo courtesy of Nina Lundholm. War (Pat Tester) the socio economics session was as di Fig. 5. IAEA Technical Cooperation project: the Caribbean team having a coordination meeting with the boss. produced by Alexandrium minutum and demonstrated that these EVs were produced in laboratory cultures and in situ bloom conditions. The EVs appeared adhered to the cell surface of A. minutum c Global HABs, Global HAB Status Reporting, and Climate Change HABs and climate change attracted a record of 139 abstracts at the Nantes Conference. Research towards predicting the impact of climate change has progressed from single factor growth experiments (e.g. temperature, pCO2) with limited HAB s Barcodes and long term changes in HAB distributions Thal-ass-ee-oh-CY-ra, Key-TOSS-er-us,Pro -toe-pear-a-DIN-ee-um, Sir-A-she-um... this might be a parody of a (very short) genetic barcode or it might be gibberish. It is in fact a sample from a list of phytoplankton genera on flashcards designed to of a highly dynamic biogeographical boundary. Taxonomic uncertainties exist in Chilean waters too, and more recently (2006) PSP was detected as far north as Bahía Mejillones in 23 S. Pre-instrumental proxies indicate climate trends on secular and longer time scales. Luminescent bands in Porites cora Unprecedented bloom of the cyano bacteria Aphanizomenon in a coastal bay of El Salvador Cyanobacteria bloom in marine, freshwater and estuarine ecosystems [1]. It is widely recognized that increased nutrient inputs in waterbodies may enhance cyanobacterial growth, resulting in harmful algal blooms [ Fig. 2. Aphanizomenon fascicles found in Bahía de Jiquilisco observed using an inverted microscope. providing video material, the Secretary of Scientific Research from the University of El Salvador for logistic support, and the Ministry of Environment and Natural Resources (MARN) for providing wate Dinoflagellate toxins recorded during an extensive coastal bloom in northern Chile Harmful algal blooms (HABs), commonly known as red tides, are primarily caused by the accelerated growth of phytoplankton due to a variety of oceanographic factors. Dinoflagellates and diatoms are often the phytoplank Table 1. Toxin concentration per sample. Toxins screened included PTX1 (pectenotoxin-1), PTX2 (pectenotoxin-2) and YTX (yessotoxin). DA (domoic acid), SPX1 (spirolide-1), GYM (gymnodimine), OA (okadaic acid), DTX1 (dinophysistoxin-1), DTX2 (dinophysistoxin-2), AZA1 (azaspiracid-1), AZA2 (azaspiracid HABs in Paradise revisited Fig. 1. Map of Rangitāhua/Kermadec Islands. Over 700 kilometres to the northeast of New Zealand are the largely uninhabited Rangitāhua/Kermadec Islands (Fig. 1). The islands fall within New Zealands exclusive economic zone and several recent expeditions have resulted in Table 1. Dinoflagellate species identified in epiphytic samples collected from Rangitāhua/Kermadec Islands, 2013-2018. Species Amphidinium carterae A.cf. massartii Coolia malayensis Gambierdiscus australes* G. honu G. polynesiensis Ostreopsis sp. 3 Prorocentrum cf. emarginatum P. hoffmanianum P Epiphytic dinoflagellates from Niue, South Pacific Ocean cal Assistance to Travellers (IAMAT) [6] suggests avoidance of reef fish over 2.7 kg and avoidance of the liver, intestine, head and roe of smaller reef fish. Gambierdiscus sp. was reported in Niue in 2008, although no molecular confirmation Fig. 2. Sampling site at Avatele Beach (left) and coastal view (right), Niue. The ICMSS is an initiative of researchers from various international institutions, including the Food and Agriculture Organization of the United Nations (FAO), related to the safety of molluscan shellfish. It seeks to be The ICES-IOC Working Group on Harmful Algal Bloom Dynamics 2019 Meeting The International Council for the Exploration of the Sea (ICES) - Intergovernmental Oceanographic Commission of UNESCO (IOC) Working Group on Harmful Algal Bloom Dynamics (WGHABD) met from the 2nd 4th April 2019. The meeting wa Intergovernmental Panel convenes to set priorities for international cooperation on mitigating the effects of Harmful Algae The Fourteenth Session of the IOC Intergovernmental Panel on Harmful Algal Blooms (IPHAB) was held at UNESCO Headquarters, Paris, from 24th to 26th April 2019. The Panel review GlobalHAB and the UN Decade of Ocean Science for Sustainable Development (2021-2030) GlobalHAB webpage (www.globalhab.info) On 1st January 2016, the 17 UN Sustainable Development Goals (SDGs) of the 2030 Agenda for Sustainable Development (adopted by world leaders in September 2015 at an historic U GlobalHAB APPLICATION FORM FOR ENDORSEMENT OF ACTIVITIES To be completed in English and send to the Chair of the GlobalHAB SSC. For further guidance consult the Chair and/or Vicechair of the GlobalHAB SSC. Date: 1. PROJECT TITLE: Planned duration of activity, from : 2. APPLICANT(S): Name and tit GlobalHAB International Coordi nated Activities Some international coordinated activities listed in the Science and Implementation Plan are being led by the GlobalHAB SSC because their implementation depends on international coordination and funding. The SSC has assumed this responsibility for their Scientists interested in the topic can contact Leonardo Guzmán (leonardo. guzman@ifop.cl) and Henrik Enevoldsen (h.enevoldsen@bio.ku.dk). May 2020 Planning is underway for a workshop on Modelling and prediction of harmful algal blooms, from event response to multi-decadal projections to be held in ISSHAs Corner The International Society for the Study of Harmful Algae (ISSHA) convened the 18th International Conference on Harmful Algae (ICHA 18), held in Nantes, France, from 21th to 26th October 2018. The ISSHA Council had two opportunistic meetings: the first before the conference on Sunday 21 Esther Garcés (Spain) Ian Jenkinson (China, France) Ingrid Sassenhagen (France, Germany) Keith Davidson (United Kingdom) Henrik Enevoldsen (Denmark) Luis Mafra (Brazil) Shauna Murray (Australia) Marta Estrada (Spain) Philipp Hess (France) Ichiro Imai (Japan) Dedmer B. Van de Wall (Netherlands) Steff logic interactions, chemical ecology and microalgal physiology. Marc Long (Best Oral Presentation) Northeast Fisheries Science Center, United States) in Milford Connecticut, studying phytoplankton physiology with Dr. Gary H. Wikfors. The work presented at ICHA2018 was part of his Ph.D. research, r She continued her research in toxicology with a thesis project on the development of new cell models of the intestinal barrier (tri-culture and co-culture with epithelial cells, goblet cells and glial enteric cells), a tool for a best evaluation of toxicity of 6 phycotoxins. This thesis is supervise Anna Godhe - In Memoriam Anna Godhe passed away on April 4th 2019 after a strenuous fight with cancer. Her untimely passing represents a big loss for the scientific community. We all remember her true passion for research, her important contributions to marine phytoplankton ecology and her open mind Forthcoming events ICHA 2020 19th ICHA October 11-16, 2020. La Paz, B.C.S. Mexico Registration will open during 2019. ISSHA members can register at special rates! Participants wishing to receive the ISSHA member rate for conference registration must join ISSHA or renew their memberships prior to t Eds-in-chief Beatriz Reguera, IEO, Vigo, Spain Eilen Bresnan, MARLAB, Marine Scotland Science, UK Regional Editors Caribbean: Ernesto Mancera jemancerap@unal.edu.co Europe: Philip Hess Philipp.Hess@ifremer.fr Mediterranean Sea: Adriana Zingone zingone@szn.it India: K.B. Padmakumar kbpadmakumar@g