Fig. 3. Gambierdiscus excentricus. Scanning electron micrographs, apical and ventral views
mostly on the left side of the cell. The
second apical plate (2) was narrow and
elongated, and located below the APC,
extending dorsally to the Po plate, and
reaching about the mid-position of the
3 plate. Plates 5 were pentagonal and
reached half of the DW axis (Fig. 4). The
thecal surface was smooth and plates
presented numerous pores of two types
(Fig. 5). Large pores and small pores
visible with the light microscope with
epifluorescence and with SEM. Analyses
of the LSU rDNA D1-D3 corroborated
previous identifications of Gambierdiscus and Ostreopsis species. The analyses of samples from 32 sampling sites
showed that O. lenticularis was widely
distributed in Sal, Sao Vicente and Boa
Vista but was not observed in Maio and
Santiago. Ostreopsis lenticularis cell
densities ranged from 50 per sample
to 1 x 106 cells per 100 cm-2. G. excentricus was distributed in all the islands
visited except for Maio. Gambierdiscus
excentricus concentrations ranged from
a few cells per sample to 150 cells per
100 cm-2.
Potentially toxic species of the genera Gambierdiscus and Ostreopsis are
reported for the first time in the Cape
Verde Islands. The presence of these
species would explain the ciguatera
cases that have been previously documented for this archipelago.
Fig. 4. Ostreopsis lenticularis. Scanning electron micrographs, apical and ventral views
Acknowledgements
were: depth (D) 84-110 μm, width
(W) 70-105 μm, and length 35-40 μm.
The thecal plate formula was: Po 4
6 6c ?s 5 1p 2. Thecal plates were
smooth with fine round to oval pores.
Apical pore plate Po was oval with a
fishhook-shaped slit and was ventrally displaced. First apical plate, 1, was
very small. Ratio between plates 2/3
and 2/4 suture lengths ranged from 2
to -2.5. Plates 1 and 6 were very small
and facing the posterior part of the cell
due to the torsion of the flagellar area
which formed a hollow. From this hollow, two flagella emerged, the longitudinal one being perpendicularly projected. The Sp was in the hypotheca, out
of the sulcus. The 2 plate was about
twice as long as wide (Fig. 3).
Ostreopsis lenticularis Y. Fukuyo
cells were broadly oval in apical and
antapical views, lenticular-shaped, biconvex, and flattened, with the cinguHARMFUL ALGAE NEWS NO. 63 / 2019
lum straight in lateral view. Measurements ranges were: depth (D) 60-105
μm, width (W) 53-74 μm and the DV/W
ratio 1.13-1.42 (mean 1.27). The thecal
plate formula was: Po 4 6 6c 4?s 5 2.
The apical pore plate Po was long and
narrow, slightly curved with the outline
of the cell. The fourth apical plate (4)
was elongated and hexagonal, located
We are grateful to the MIRPURI FOUNDATION for their financial support of
the expedition to Isla de la Sal (Sal Island) in July-August 2019
References
1. Wells M. et al 2015. Harmful Algae
1;49:68-93
2. Hallegraeff GM 2010. J Phycol 46:
220235
3. Silva ES 1956. Bull LI. F. A. N. XVIII, série
A. T. XVIII, sér. A, n, 2, pp. 335-371
4. Fraga S et al 2014. Protist 165: 839-853.
Authors
Emilio Soler Onís & Juan Fernández Zabala,
Observatorio Canario de HABs, FCPCT-ULPGC, Parque Científico Tecnológico Marino
de Taliarte. C/ Miramar, 121. 35214 Taliarte,
Las Palmas. Spain
Fig. 5. Ostreopsis lenticularis. Epifluorescence micrograph showing detail of the thecal surface with two kinds of thecal pores.
Ana S Ramirez, Facultad de Veterinaria,
ULPGC, Campus Universitario Cardones de
Arucas, 35413 Arucas, Spain
Corresponding author: esoler@fcpct.ulpgc.es
9
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