Fig. 3. Raphidophyte 2 from a culture in
1974. Motile cell, ventral view 11.5 μm long.
reproduced here at a smaller size (Fig.
4) and are interpreted (in the figure
caption) by the author.
Photographs of motile cells under
stress received from Nicky Haigh (Mi-
crothalassia) (not shown here) reveal
that the extrusomes are flexible and not
the impediment to motility that they
appear to be in Fig. 4 #2. They are more
evenly distributed and more uniform
in width except for constricted distal
ends. Nickys cells have a tubular pos-
terior invagination on their ventral side
and a corresponding shallow recess on
their dorsal, which may explain why
the cells in the 1990 bloom split open
in the fixative (Fig. 2 #1). Those cells
were growing fast when they were col-
Fig. 4. Flagellate X from Droop 1979. Cell size about 20 μm. #1 Ventral face,
anterior end down; note the thin and overlapping chloroplasts. #2 The same
cell under stress, its ejected extrusomes apparently unfilled. #3 A cell shrunk
by the apparent transfer of mucilage to the extrusomes, which have released
it and dispersed (#1-3 captions are interpretations from the author).
lected. Slow-growing cells have round
chloroplasts in Lugols-fixed material,
where they can resemble Heterosigma
akashiwo (Fig. 2 #6). Although the two
species have different water tempera-
ture requirements, they can both be
present at high concentrations in the
same bloom in BC coastal waters 2,4.
Acknowledgements
I thank Patricia Smith for carrying out
the OsO4 test on the Vicicitus and Nicky
Haigh for the missing information.
References
1. Waters R 1992. Aquaculture Association
of BC Newsletter 8 (2): 6-10, out of print
IOC-IAEA guide for designing and implementing a plan to monitor
toxin-producing microalgae
The IOC and IAEA are proud to an-
nounce the publication of IOC Manu-
als & Guides no 59
This manual is intended as an introduc-
tion to basic analytical techniques that
can be applied when designing a stand-
ard sampling protocol for both plank-
tonic and benthic microalgae (and asso-
10
ciated environmental conditions) and
vectors of biotoxins (shellfish and fish).
This standardization of methods will en-
able more robust data comparisons be-
tween countries and will yield improved
risk assessments of potentially toxic
HABs events. http://unesdoc.unesco.
org/images/0021/002145/214510e.pdf
2. Waters RE 2010. Harmful Algae News
41:3-5
3. Waters RE et al 1992. Can Tech Rep
Hydrogr Ocean Sci 137, 59 pp
4. Forbes JR & RE Waters 1993. Can Data
Rep Hydrogr Ocean Sci 117 Vol 1: 1979-
1984, 212 pp Vol 2: 1985-1989, 247 pp
5. Chang FH et al 2012. Phycologia 51):
403-420
6. Taylor FJR, Haigh R & TF Sutherland
1991. In: Forbes JR (ed) 1991. Can Tech
Rep Hydrogr Ocean Sci 135:23-28
7. Droop MR et al 1979. In: Tett P (ed)
1980. Scottish Mar Biol Assoc Internal
Report 25: 88-97
Author
Rosemary Waters, Institute of Ocean
Sciences, Sidney, BC V8L 4B2, Canada
Email: richardswatersbtinternet.com
Intergovernm
ental
Oceanogra
ph
Commission ic
Manuals and
Guides
59
GUIDE FO
R DE
A PL AN TO SIGNING AND IMPL
EMENTIN
MONITOR
G
TOXIN-PR
ODUCING
MICROALG
AE
Second Ed
ition
Beatriz Re
guera, Ro
Silvia Mé
salba Alo
ndez and
nso, Ánge
Marie -Yasm
l Mo
ine Dechrao reira,
ui Bottein
HARMFUL ALGAE NEWS NO. 56 / 2017
Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 56 - March 2017 www.ioc-unesco.org/hab Is Gambierdiscus expanding its geographic range in the Pacific region? Content HAB events and toxin effects Gambierdiscus distribution in the Pacific . ...................................
Table 1: Geographic occurrence and toxin production (as determined by LCMS/MS) of Gambierdiscus species in the Pacific region. Species reported in the Pacific region G. australes G. balechii G. belizeanus G. caribaeus G. carpenteri G. cheloniae G. lapillus G. pacificus G. polynesiensis G. scabrosus
Fig. 2. Scanning electron micrographs of cells of Gambierdiscus sp., isolate CAWD242, from the Kermadec Islands: (A) view of cingulum and apical pore, (B) apical pore complex, (C) antapi- cal hypotheca. this will bring the number of Gambier- discus species to fifteen. A related species, originally d
lished data). The role of MTXs in ciguat- era fish poisoning is uncertain and due to its low oral toxicity may only play a small part in toxic events 21. A range of cell-based assays is avail- able to detect CFP-related toxins, includ- ing receptor binding (RBA), Ca 2+ flux, N2A and haemolytic assay
Toxin production in lab-rat-diatoms (e.g. Pseudo-nitzschia) in the presence of copepods From only 15 species in 1965, currently 49 Pseudo-nitzschia species have been described, of which 22 have the abil- ity to produce the toxin domoic acid (DA), along with two Nitzschia species 1. Both numbers are
Apart from increasing toxin produc- tion in already toxic species/strains, presence of grazers may even induce toxin production in species previously considered non-toxic, as shown for P. obtusa 3. It is therefore relevant to consider that all Pseudo-nitzschia spe- cies might have the potential to p
Ecology of Alexandrium spp. in the Strait of Georgia, British Columbia, Canada 2015 Fig. 1 Citizen Science sampling stations in the Strait of Georgia, Canada in 2015. Fig. 2. Citizen Science sampling areas in the Strait of Georgia, Canada in 2015. Coastal waters of British Columbia (BC), Canada have
Table 1. Number of water samples (N) collected by the Citizen Science Program, frequency of samples with Alexandrium spp. presence (Alex. +), and number of water samples with Alexan- drium spp. cells present by month, Strait of Georgia 2015 (n/a - no samples were collected). Area Ladysmith Cowichan
A clarification of three unnamed raphidophytes previously described from British Columbia gated motile cell with very similar chlo- roplasts and the oval Lugol-fixed cell in Fig. 2 #3 were probably equivalent to the long cells formed by V. globosus in stationary growth (see 5 Figs. 7 & 8). Raphidoph
Fig. 3. Raphidophyte 2 from a culture in 1974. Motile cell, ventral view 11.5 μm long. reproduced here at a smaller size (Fig. 4) and are interpreted (in the figure caption) by the author. Photographs of motile cells under stress received from Nicky Haigh (Mi- crothalassia) (not shown here) reveal t
Autumnal algal bloom succession in northern coasts of Gulf of Oman Fig. 1. Map of the study area The composition of harmful algal blooms is determined by the diversity and abundance of different species pre- sent. Temporal fluctuations in phyto- plankton populations can lead to a suc- cession of dif
Table 1. Maximal mean density of the observed bloom species and physical-chemical properties of the near-shore waters during autumnal algal blooms in the northern Gulf of Oman Date 26 October 2016 04 November 2016 09 November 2016 29 November 2016 03 December 2016 Species/ Genus Alexandrium sp. Pror
Nodularin accumulation in New Zealand shortfin eel from Lake Forsyth/Te Wairewa in 1866 2 and, from the 1940s onwards, open- ings have been carried out regularly. In 2008, the rūnanga attempted to create a permanent opening by constructing a 900 m canal and 300 m groyne at the eastern end of the bea
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The Scottish Coastal Observatory Scotlands coastal environment is sub- ject to inherent variability resulting from short term processes such as tides and weather, the seasonal cycle, multi- annual cycles such as the North Atlantic Oscillation and larger scale influences such as climate change and oc
The RedFANnetwork: a working group to deal with HABs in Mexico In October 2014 the network RedFAN (from the acronym in Spanish: Red temática sobre Florecimientos Algales Nocivos) was created with the financial support of the Mexican National Coun- cil of Science and Technology (CONA- CyT). This netw
Caribbean Ciguatera Experts Discuss a Risk Management System for the IOCARIBE Region Over the past three decades the inci- dence of ciguatera has increased in the Caribbean countries by about 32 1. Despite the evidence of toxic events and their potential consequences on public health, fisheries and
IOC/WESTPAC Scientists Shape the Future of Regional HAB Research More than twenty experts from coun- tries in the Western Pacific gathered in Nha Trang, Vietnam, 19-22 December 2016 to foster cooperation and define research priorities for Harmful Algal Blooms (HABs) in the region (Fig. 1). The East
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In memory of Paul Harrison With great sorrow, we learned of the recent unexpected passing of our col- league, mentor and friend Paul J. Har- rison. Among his many contributions, Paul was a founding member of the Sci- entific Committee on Oceanic Research (SCOR) working Group 137 SCOR WG 137 Global
supply, marine resources, climate change impacts and environmental protection. His voice and perspec- tive will be sorely absent. Paul will be greatly missed by those he touched, but especially by Victoria, his wife of 51 years, and their children Rachel, Richard and Christina. A scholarship fund th
11th International Conference on Modern and Fossil Dinoflagellates The University of Bordeaux (France) is the organiser of the 11 th International Conference on Modern and Fossil Dino- flagellates, DINO11, which will be held from 17 to 21 July 2017 at Bordeaux, France. This series of conference was
IOC Qualifications in Identification and Enumeration of Harmful Marine Microalgae A now traditional IOC course on identi- fication of harmful marine microalgae, including optional workshops on enu- meration and culture techniques. The 2017 course will be held from 6 to 19 August at the IOC Science a
Taxonomic Note Oceanography special issue Alexandrium catenella vs. A. fundyense The Nomenclature Committee for Algae has decided (Prudhomme van Reine, 2017) that the name Gonyaulax catenel- la (Alexandrium catenella) should not be rejected and that A. fundyense and A. catenella are conspecific havi