Fig. 3. Raphidophyte 2 from a culture in 1974. Motile cell, ventral view 11.5 μm long. reproduced here at a smaller size (Fig. 4) and are interpreted (in the figure caption) by the author. Photographs of motile cells under stress received from Nicky Haigh (Mi- crothalassia) (not shown here) reveal that the extrusomes are flexible and not the impediment to motility that they appear to be in Fig. 4 #2. They are more evenly distributed and more uniform in width except for constricted distal ends. Nickys cells have a tubular pos- terior invagination on their ventral side and a corresponding shallow recess on their dorsal, which may explain why the cells in the 1990 bloom split open in the fixative (Fig. 2 #1). Those cells were growing fast when they were col- Fig. 4. Flagellate X from Droop 1979. Cell size about 20 μm. #1 Ventral face, anterior end down; note the thin and overlapping chloroplasts. #2 The same cell under stress, its ejected extrusomes apparently unfilled. #3 A cell shrunk by the apparent transfer of mucilage to the extrusomes, which have released it and dispersed (#1-3 captions are interpretations from the author). lected. Slow-growing cells have round chloroplasts in Lugols-fixed material, where they can resemble Heterosigma akashiwo (Fig. 2 #6). Although the two species have different water tempera- ture requirements, they can both be present at high concentrations in the same bloom in BC coastal waters 2,4. Acknowledgements I thank Patricia Smith for carrying out the OsO4 test on the Vicicitus and Nicky Haigh for the missing information. References 1. Waters R 1992. Aquaculture Association of BC Newsletter 8 (2): 6-10, out of print IOC-IAEA guide for designing and implementing a plan to monitor toxin-producing microalgae The IOC and IAEA are proud to an- nounce the publication of IOC Manu- als & Guides no 59 This manual is intended as an introduc- tion to basic analytical techniques that can be applied when designing a stand- ard sampling protocol for both plank- tonic and benthic microalgae (and asso- 10 ciated environmental conditions) and vectors of biotoxins (shellfish and fish). This standardization of methods will en- able more robust data comparisons be- tween countries and will yield improved risk assessments of potentially toxic HABs events. http://unesdoc.unesco. org/images/0021/002145/214510e.pdf 2. Waters RE 2010. Harmful Algae News 41:3-5 3. Waters RE et al 1992. Can Tech Rep Hydrogr Ocean Sci 137, 59 pp 4. Forbes JR & RE Waters 1993. Can Data Rep Hydrogr Ocean Sci 117 Vol 1: 1979- 1984, 212 pp Vol 2: 1985-1989, 247 pp 5. Chang FH et al 2012. Phycologia 51): 403-420 6. Taylor FJR, Haigh R & TF Sutherland 1991. In: Forbes JR (ed) 1991. Can Tech Rep Hydrogr Ocean Sci 135:23-28 7. Droop MR et al 1979. In: Tett P (ed) 1980. Scottish Mar Biol Assoc Internal Report 25: 88-97 Author Rosemary Waters, Institute of Ocean Sciences, Sidney, BC V8L 4B2, Canada Email: richardswatersbtinternet.com Intergovernm ental Oceanogra ph Commission ic Manuals and Guides 59 GUIDE FO R DE A PL AN TO SIGNING AND IMPL EMENTIN MONITOR G TOXIN-PR ODUCING MICROALG AE Second Ed ition Beatriz Re guera, Ro Silvia Mé salba Alo ndez and nso, Ánge Marie -Yasm l Mo ine Dechrao reira, ui Bottein HARMFUL ALGAE NEWS NO. 56 / 2017 Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 56 - March 2017 www.ioc-unesco.org/hab Is Gambierdiscus expanding its geographic range in the Pacific region? Content HAB events and toxin effects Gambierdiscus distribution in the Pacific . ................................... Table 1: Geographic occurrence and toxin production (as determined by LCMS/MS) of Gambierdiscus species in the Pacific region. Species reported in the Pacific region G. australes G. balechii G. belizeanus G. caribaeus G. carpenteri G. cheloniae G. lapillus G. pacificus G. polynesiensis G. scabrosus Fig. 2. Scanning electron micrographs of cells of Gambierdiscus sp., isolate CAWD242, from the Kermadec Islands: (A) view of cingulum and apical pore, (B) apical pore complex, (C) antapi- cal hypotheca. this will bring the number of Gambier- discus species to fifteen. A related species, originally d lished data). The role of MTXs in ciguat- era fish poisoning is uncertain and due to its low oral toxicity may only play a small part in toxic events 21. A range of cell-based assays is avail- able to detect CFP-related toxins, includ- ing receptor binding (RBA), Ca 2+ flux, N2A and haemolytic assay Toxin production in lab-rat-diatoms (e.g. Pseudo-nitzschia) in the presence of copepods From only 15 species in 1965, currently 49 Pseudo-nitzschia species have been described, of which 22 have the abil- ity to produce the toxin domoic acid (DA), along with two Nitzschia species 1. Both numbers are Apart from increasing toxin produc- tion in already toxic species/strains, presence of grazers may even induce toxin production in species previously considered non-toxic, as shown for P. obtusa 3. It is therefore relevant to consider that all Pseudo-nitzschia spe- cies might have the potential to p Ecology of Alexandrium spp. in the Strait of Georgia, British Columbia, Canada 2015 Fig. 1 Citizen Science sampling stations in the Strait of Georgia, Canada in 2015. Fig. 2. Citizen Science sampling areas in the Strait of Georgia, Canada in 2015. Coastal waters of British Columbia (BC), Canada have Table 1. Number of water samples (N) collected by the Citizen Science Program, frequency of samples with Alexandrium spp. presence (Alex. +), and number of water samples with Alexan- drium spp. cells present by month, Strait of Georgia 2015 (n/a - no samples were collected). Area Ladysmith Cowichan A clarification of three unnamed raphidophytes previously described from British Columbia gated motile cell with very similar chlo- roplasts and the oval Lugol-fixed cell in Fig. 2 #3 were probably equivalent to the long cells formed by V. globosus in stationary growth (see 5 Figs. 7 & 8). Raphidoph Fig. 3. Raphidophyte 2 from a culture in 1974. Motile cell, ventral view 11.5 μm long. reproduced here at a smaller size (Fig. 4) and are interpreted (in the figure caption) by the author. Photographs of motile cells under stress received from Nicky Haigh (Mi- crothalassia) (not shown here) reveal t Autumnal algal bloom succession in northern coasts of Gulf of Oman Fig. 1. Map of the study area The composition of harmful algal blooms is determined by the diversity and abundance of different species pre- sent. Temporal fluctuations in phyto- plankton populations can lead to a suc- cession of dif Table 1. Maximal mean density of the observed bloom species and physical-chemical properties of the near-shore waters during autumnal algal blooms in the northern Gulf of Oman Date 26 October 2016 04 November 2016 09 November 2016 29 November 2016 03 December 2016 Species/ Genus Alexandrium sp. Pror Nodularin accumulation in New Zealand shortfin eel from Lake Forsyth/Te Wairewa in 1866 2 and, from the 1940s onwards, open- ings have been carried out regularly. In 2008, the rūnanga attempted to create a permanent opening by constructing a 900 m canal and 300 m groyne at the eastern end of the bea Fig. 2. Nodularia spumigena from Lake Forsyth/Te Wairewa at 400 magnification (A) and blooms of the cyanobacterium at the lake in 2006 (B). Fig. 3. Eel fishing channels at Lake Forsyth/Te Wairewa (A) and members of the rūnanga of Wairewa catching eels in the channels by gaffing (B; photo provided by The Scottish Coastal Observatory Scotlands coastal environment is sub- ject to inherent variability resulting from short term processes such as tides and weather, the seasonal cycle, multi- annual cycles such as the North Atlantic Oscillation and larger scale influences such as climate change and oc The RedFANnetwork: a working group to deal with HABs in Mexico In October 2014 the network RedFAN (from the acronym in Spanish: Red temática sobre Florecimientos Algales Nocivos) was created with the financial support of the Mexican National Coun- cil of Science and Technology (CONA- CyT). This netw Caribbean Ciguatera Experts Discuss a Risk Management System for the IOCARIBE Region Over the past three decades the inci- dence of ciguatera has increased in the Caribbean countries by about 32 1. Despite the evidence of toxic events and their potential consequences on public health, fisheries and IOC/WESTPAC Scientists Shape the Future of Regional HAB Research More than twenty experts from coun- tries in the Western Pacific gathered in Nha Trang, Vietnam, 19-22 December 2016 to foster cooperation and define research priorities for Harmful Algal Blooms (HABs) in the region (Fig. 1). The East ing the ongoing HAB research and ca- pacity building efforts at these levels. Dr. Po Teen Lim presented the science and implementation plans of the IOC- SCOR sponsored new GlobalHAB re- search program (Fig. 3). It was widely recognized that national and regional efforts are complementary to global e In memory of Paul Harrison With great sorrow, we learned of the recent unexpected passing of our col- league, mentor and friend Paul J. Har- rison. Among his many contributions, Paul was a founding member of the Sci- entific Committee on Oceanic Research (SCOR) working Group 137 SCOR WG 137 Global supply, marine resources, climate change impacts and environmental protection. His voice and perspec- tive will be sorely absent. Paul will be greatly missed by those he touched, but especially by Victoria, his wife of 51 years, and their children Rachel, Richard and Christina. A scholarship fund th 11th International Conference on Modern and Fossil Dinoflagellates The University of Bordeaux (France) is the organiser of the 11 th International Conference on Modern and Fossil Dino- flagellates, DINO11, which will be held from 17 to 21 July 2017 at Bordeaux, France. This series of conference was IOC Qualifications in Identification and Enumeration of Harmful Marine Microalgae A now traditional IOC course on identi- fication of harmful marine microalgae, including optional workshops on enu- meration and culture techniques. The 2017 course will be held from 6 to 19 August at the IOC Science a Taxonomic Note Oceanography special issue Alexandrium catenella vs. A. fundyense The Nomenclature Committee for Algae has decided (Prudhomme van Reine, 2017) that the name Gonyaulax catenel- la (Alexandrium catenella) should not be rejected and that A. fundyense and A. catenella are conspecific havi