Table 1. Number of water samples (N) collected by the Citizen Science Program, frequency of
samples with Alexandrium spp. presence (Alex. +), and number of water samples with Alexan-
drium spp. cells present by month, Strait of Georgia 2015 (n/a - no samples were collected).
Area
Ladysmith
Cowichan Bay
Lund
Irvine Sechel
Baynes sound
Victoria
Powell River
Campbell River
Nanaimo
Samples
(N) ()
Alex. + Mar Apr
27 63 n/a n/a
83 11 1 162 4 1
149
128
98
99
144
107
40
Steveston
26
13
9
6
2
2
0
1
2
May
1
10
3
1
1
1
1
1
Table 2. Descriptive statistics of dissolved nutrients values
(μmol/L) at 2 and 20 m when Alexandrium spp. cells were pre-
sent at 0 m in 1 mL of the sample (N=20 samples)
Min
Max
Mean
SE
Var
Nutrients at 0 m
NO 3
SiO 3
PO 4
+ NO 2
0,03
11,30
0,24
19,31 45,98 1,81
27,67 101,33 0,17
4,22
1,18
24,17
2,25
0,84
0,09
Nutrients at 20 m
NO 3
SiO 3
PO 4
+ NO 2
10,70
8,97 1,31
2,16 0,07
24,97 47,87
25,65 93,08
16,97
1,13
34,15
2,27
1,77
0,10
Fig. 3. Average density of Alexandrium spp. at the Ladysmith
sites (N=5) from June to September, 2015
Fig. 4. Average density of Alexandrium spp. at the Cowichan Bay
sites (N=13) from July to September, 2015
Fig. 5. Temperature and salinity values at 0.5 m when Alexandri-
um spp. cells were present at 0 m in 1 mL of the sample (N=48)
8
Jun
10
n/a
6
4
2
2
2
1
Jul Aug Sep
4 n/a 2
3 4
18
5
3
6
1
1
4
1
1
3
a range of 16-19 C and
26-28 ppt.
Nutrients. There were
only 20 cases when
dissolved nutrient sam-
ples were collected (at
2 and 20 m) along with
phytoplankton
sam-
ples containing at least
1 cell mL -1 of Alexan-
drium spp. (at 0 m) and
where nutrient values
were above zero within
analytical uncertainty.
Nutrient values varied
dramatically (Table 3)
and due to the small
sample size, no clear
pattern between nutrient
values and Alexandrium
spp. presence was estab-
lished. An interesting
observation was that out
of these 20 cases, there
were only 2 with high
Alexandrium spp. den-
sity (8 and 10 cells
mL -1 ; the remaining 18
cases had 1-3 cells mL -
1 ) and in both of these
cases, nitrate and nitrite
values (at 2 m) were rel-
atively high (7.44 and
6.7 μmoles L -1 , respec-
tively).
The year of 2015
was unusually warm
due to El Niño. In the
Strait of Georgia the
majority of the total
phytoplankton
bio-
mass was comprised
of diatoms, while the
dinoflagellate contri-
bution was very low
and silicoflagellates and raphidophytes
were almost absent (Pacific Salmon
Foundation, internal data). Hence, data
on Alexandrium spp. distribution, abun-
dance and environmental preferences
reported here are limited and represent
the behaviour of such harmful algae in
an unusual year. The most important
observation of the Alexandrium spp. dis-
tribution in the Strait of Georgia in 2015
was that location and season were the
most important factors in Alexandrium
spp. presence and absence. The Citizen
Science Program supported by PSF is
continuing the collection and analysis
of samples through 2016 and 2017.
This anticipated 3 year continuation of
data collection will be highly beneficial
in understanding the local ecology of
PSP-causing species.
Acknowledgments
We are greatly appreciative to the nu-
merous Pacific Salmon Foundation
donors whose contributions fund the
Salish Sea Marine Survival Project
www.marinesurvivalproject.com and
the Sitka Foundation for their gener-
ous support of the Strait of Georgia
Data Centre www.sogdatacentre.ca.
We thank all the Citizen Scientist crews
and the Stzuminus First Nations for
diligently collecting samples across the
Strait of Georgia. Thank you to Benja-
min Skinner for producing maps for this
report. Furthermore, we would like to
acknowledge that the idea of preparing
this report was conceived at the Marine
Biotoxin Workshop 2016 organized by
the BC Centre for Disease Control.
References
1. Vancouver G. 1798. In: Robinson GG & J
Robinson (eds) Vol. 2, 4 th book, chapter
2 (Paternoster-Row and J Edwards,
London) pp 260287
2. Fisheries and Oceans Canada 2017.
Shellfish Contamination in the Pacific
Region. http://www.pac.dfo-mpo.gc.ca/
fm-gp/contamination/index-eng.html
3. Taylor FJR & PJ Harrison 2002. In:
Taylor FJR & VM Trainer (eds) Harmful
algal blooms in the PICES region of the
North Pacific. PICES Rep. 23, pp 7788
Authors
Svetlana Esenkulova, Isabel Pearsall & Colin
Novak, Pacific Salmon Foundation, 1682-300
West 7 th Avenue, Vancouver, BC, V6J 4S6,
Canada
Email corresponding author: svesenuvic.ca
HARMFUL ALGAE NEWS NO. 56 / 2017
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Table 1: Geographic occurrence and toxin production (as determined by LCMS/MS) of Gambierdiscus species in the Pacific region. Species reported in the Pacific region G. australes G. balechii G. belizeanus G. caribaeus G. carpenteri G. cheloniae G. lapillus G. pacificus G. polynesiensis G. scabrosus
Fig. 2. Scanning electron micrographs of cells of Gambierdiscus sp., isolate CAWD242, from the Kermadec Islands: (A) view of cingulum and apical pore, (B) apical pore complex, (C) antapi- cal hypotheca. this will bring the number of Gambier- discus species to fifteen. A related species, originally d
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Table 1. Number of water samples (N) collected by the Citizen Science Program, frequency of samples with Alexandrium spp. presence (Alex. +), and number of water samples with Alexan- drium spp. cells present by month, Strait of Georgia 2015 (n/a - no samples were collected). Area Ladysmith Cowichan
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