Table 1. Number of water samples (N) collected by the Citizen Science Program, frequency of samples with Alexandrium spp. presence (Alex. +), and number of water samples with Alexan- drium spp. cells present by month, Strait of Georgia 2015 (n/a - no samples were collected). Area Ladysmith Cowichan Bay Lund Irvine Sechel Baynes sound Victoria Powell River Campbell River Nanaimo Samples (N) () Alex. + Mar Apr 27 63 n/a n/a 83 11 1 162 4 1 149 128 98 99 144 107 40 Steveston 26 13 9 6 2 2 0 1 2 May 1 10 3 1 1 1 1 1 Table 2. Descriptive statistics of dissolved nutrients values (μmol/L) at 2 and 20 m when Alexandrium spp. cells were pre- sent at 0 m in 1 mL of the sample (N=20 samples) Min Max Mean SE Var Nutrients at 0 m NO 3 SiO 3 PO 4 + NO 2 0,03 11,30 0,24 19,31 45,98 1,81 27,67 101,33 0,17 4,22 1,18 24,17 2,25 0,84 0,09 Nutrients at 20 m NO 3 SiO 3 PO 4 + NO 2 10,70 8,97 1,31 2,16 0,07 24,97 47,87 25,65 93,08 16,97 1,13 34,15 2,27 1,77 0,10 Fig. 3. Average density of Alexandrium spp. at the Ladysmith sites (N=5) from June to September, 2015 Fig. 4. Average density of Alexandrium spp. at the Cowichan Bay sites (N=13) from July to September, 2015 Fig. 5. Temperature and salinity values at 0.5 m when Alexandri- um spp. cells were present at 0 m in 1 mL of the sample (N=48) 8 Jun 10 n/a 6 4 2 2 2 1 Jul Aug Sep 4 n/a 2 3 4 18 5 3 6 1 1 4 1 1 3 a range of 16-19 C and 26-28 ppt. Nutrients. There were only 20 cases when dissolved nutrient sam- ples were collected (at 2 and 20 m) along with phytoplankton sam- ples containing at least 1 cell mL -1 of Alexan- drium spp. (at 0 m) and where nutrient values were above zero within analytical uncertainty. Nutrient values varied dramatically (Table 3) and due to the small sample size, no clear pattern between nutrient values and Alexandrium spp. presence was estab- lished. An interesting observation was that out of these 20 cases, there were only 2 with high Alexandrium spp. den- sity (8 and 10 cells mL -1 ; the remaining 18 cases had 1-3 cells mL - 1 ) and in both of these cases, nitrate and nitrite values (at 2 m) were rel- atively high (7.44 and 6.7 μmoles L -1 , respec- tively). The year of 2015 was unusually warm due to El Niño. In the Strait of Georgia the majority of the total phytoplankton bio- mass was comprised of diatoms, while the dinoflagellate contri- bution was very low and silicoflagellates and raphidophytes were almost absent (Pacific Salmon Foundation, internal data). Hence, data on Alexandrium spp. distribution, abun- dance and environmental preferences reported here are limited and represent the behaviour of such harmful algae in an unusual year. The most important observation of the Alexandrium spp. dis- tribution in the Strait of Georgia in 2015 was that location and season were the most important factors in Alexandrium spp. presence and absence. The Citizen Science Program supported by PSF is continuing the collection and analysis of samples through 2016 and 2017. This anticipated 3 year continuation of data collection will be highly beneficial in understanding the local ecology of PSP-causing species. Acknowledgments We are greatly appreciative to the nu- merous Pacific Salmon Foundation donors whose contributions fund the Salish Sea Marine Survival Project www.marinesurvivalproject.com and the Sitka Foundation for their gener- ous support of the Strait of Georgia Data Centre www.sogdatacentre.ca. We thank all the Citizen Scientist crews and the Stzuminus First Nations for diligently collecting samples across the Strait of Georgia. Thank you to Benja- min Skinner for producing maps for this report. Furthermore, we would like to acknowledge that the idea of preparing this report was conceived at the Marine Biotoxin Workshop 2016 organized by the BC Centre for Disease Control. References 1. Vancouver G. 1798. In: Robinson GG & J Robinson (eds) Vol. 2, 4 th book, chapter 2 (Paternoster-Row and J Edwards, London) pp 260287 2. Fisheries and Oceans Canada 2017. Shellfish Contamination in the Pacific Region. http://www.pac.dfo-mpo.gc.ca/ fm-gp/contamination/index-eng.html 3. Taylor FJR & PJ Harrison 2002. In: Taylor FJR & VM Trainer (eds) Harmful algal blooms in the PICES region of the North Pacific. PICES Rep. 23, pp 7788 Authors Svetlana Esenkulova, Isabel Pearsall & Colin Novak, Pacific Salmon Foundation, 1682-300 West 7 th Avenue, Vancouver, BC, V6J 4S6, Canada Email corresponding author: svesenuvic.ca HARMFUL ALGAE NEWS NO. 56 / 2017 Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 56 - March 2017 www.ioc-unesco.org/hab Is Gambierdiscus expanding its geographic range in the Pacific region? Content HAB events and toxin effects Gambierdiscus distribution in the Pacific . ................................... Table 1: Geographic occurrence and toxin production (as determined by LCMS/MS) of Gambierdiscus species in the Pacific region. Species reported in the Pacific region G. australes G. balechii G. belizeanus G. caribaeus G. carpenteri G. cheloniae G. lapillus G. pacificus G. polynesiensis G. scabrosus Fig. 2. Scanning electron micrographs of cells of Gambierdiscus sp., isolate CAWD242, from the Kermadec Islands: (A) view of cingulum and apical pore, (B) apical pore complex, (C) antapi- cal hypotheca. this will bring the number of Gambier- discus species to fifteen. A related species, originally d lished data). The role of MTXs in ciguat- era fish poisoning is uncertain and due to its low oral toxicity may only play a small part in toxic events 21. A range of cell-based assays is avail- able to detect CFP-related toxins, includ- ing receptor binding (RBA), Ca 2+ flux, N2A and haemolytic assay Toxin production in lab-rat-diatoms (e.g. Pseudo-nitzschia) in the presence of copepods From only 15 species in 1965, currently 49 Pseudo-nitzschia species have been described, of which 22 have the abil- ity to produce the toxin domoic acid (DA), along with two Nitzschia species 1. Both numbers are Apart from increasing toxin produc- tion in already toxic species/strains, presence of grazers may even induce toxin production in species previously considered non-toxic, as shown for P. obtusa 3. It is therefore relevant to consider that all Pseudo-nitzschia spe- cies might have the potential to p Ecology of Alexandrium spp. in the Strait of Georgia, British Columbia, Canada 2015 Fig. 1 Citizen Science sampling stations in the Strait of Georgia, Canada in 2015. Fig. 2. Citizen Science sampling areas in the Strait of Georgia, Canada in 2015. Coastal waters of British Columbia (BC), Canada have Table 1. Number of water samples (N) collected by the Citizen Science Program, frequency of samples with Alexandrium spp. presence (Alex. +), and number of water samples with Alexan- drium spp. cells present by month, Strait of Georgia 2015 (n/a - no samples were collected). Area Ladysmith Cowichan A clarification of three unnamed raphidophytes previously described from British Columbia gated motile cell with very similar chlo- roplasts and the oval Lugol-fixed cell in Fig. 2 #3 were probably equivalent to the long cells formed by V. globosus in stationary growth (see 5 Figs. 7 & 8). Raphidoph Fig. 3. Raphidophyte 2 from a culture in 1974. Motile cell, ventral view 11.5 μm long. reproduced here at a smaller size (Fig. 4) and are interpreted (in the figure caption) by the author. Photographs of motile cells under stress received from Nicky Haigh (Mi- crothalassia) (not shown here) reveal t Autumnal algal bloom succession in northern coasts of Gulf of Oman Fig. 1. Map of the study area The composition of harmful algal blooms is determined by the diversity and abundance of different species pre- sent. Temporal fluctuations in phyto- plankton populations can lead to a suc- cession of dif Table 1. Maximal mean density of the observed bloom species and physical-chemical properties of the near-shore waters during autumnal algal blooms in the northern Gulf of Oman Date 26 October 2016 04 November 2016 09 November 2016 29 November 2016 03 December 2016 Species/ Genus Alexandrium sp. Pror Nodularin accumulation in New Zealand shortfin eel from Lake Forsyth/Te Wairewa in 1866 2 and, from the 1940s onwards, open- ings have been carried out regularly. In 2008, the rūnanga attempted to create a permanent opening by constructing a 900 m canal and 300 m groyne at the eastern end of the bea Fig. 2. Nodularia spumigena from Lake Forsyth/Te Wairewa at 400 magnification (A) and blooms of the cyanobacterium at the lake in 2006 (B). Fig. 3. Eel fishing channels at Lake Forsyth/Te Wairewa (A) and members of the rūnanga of Wairewa catching eels in the channels by gaffing (B; photo provided by The Scottish Coastal Observatory Scotlands coastal environment is sub- ject to inherent variability resulting from short term processes such as tides and weather, the seasonal cycle, multi- annual cycles such as the North Atlantic Oscillation and larger scale influences such as climate change and oc The RedFANnetwork: a working group to deal with HABs in Mexico In October 2014 the network RedFAN (from the acronym in Spanish: Red temática sobre Florecimientos Algales Nocivos) was created with the financial support of the Mexican National Coun- cil of Science and Technology (CONA- CyT). This netw Caribbean Ciguatera Experts Discuss a Risk Management System for the IOCARIBE Region Over the past three decades the inci- dence of ciguatera has increased in the Caribbean countries by about 32 1. Despite the evidence of toxic events and their potential consequences on public health, fisheries and IOC/WESTPAC Scientists Shape the Future of Regional HAB Research More than twenty experts from coun- tries in the Western Pacific gathered in Nha Trang, Vietnam, 19-22 December 2016 to foster cooperation and define research priorities for Harmful Algal Blooms (HABs) in the region (Fig. 1). The East ing the ongoing HAB research and ca- pacity building efforts at these levels. Dr. Po Teen Lim presented the science and implementation plans of the IOC- SCOR sponsored new GlobalHAB re- search program (Fig. 3). It was widely recognized that national and regional efforts are complementary to global e In memory of Paul Harrison With great sorrow, we learned of the recent unexpected passing of our col- league, mentor and friend Paul J. Har- rison. Among his many contributions, Paul was a founding member of the Sci- entific Committee on Oceanic Research (SCOR) working Group 137 SCOR WG 137 Global supply, marine resources, climate change impacts and environmental protection. His voice and perspec- tive will be sorely absent. Paul will be greatly missed by those he touched, but especially by Victoria, his wife of 51 years, and their children Rachel, Richard and Christina. A scholarship fund th 11th International Conference on Modern and Fossil Dinoflagellates The University of Bordeaux (France) is the organiser of the 11 th International Conference on Modern and Fossil Dino- flagellates, DINO11, which will be held from 17 to 21 July 2017 at Bordeaux, France. This series of conference was IOC Qualifications in Identification and Enumeration of Harmful Marine Microalgae A now traditional IOC course on identi- fication of harmful marine microalgae, including optional workshops on enu- meration and culture techniques. The 2017 course will be held from 6 to 19 August at the IOC Science a Taxonomic Note Oceanography special issue Alexandrium catenella vs. A. fundyense The Nomenclature Committee for Algae has decided (Prudhomme van Reine, 2017) that the name Gonyaulax catenel- la (Alexandrium catenella) should not be rejected and that A. fundyense and A. catenella are conspecific havi