lished data). The role of MTXs in ciguat-
era fish poisoning is uncertain and due
to its low oral toxicity may only play a
small part in toxic events 21.
A range of cell-based assays is avail-
able to detect CFP-related toxins, includ-
ing receptor binding (RBA), Ca 2+ flux,
N2A and haemolytic assays 21 as well as
whole animal assays (for example, mouse
and larval bioassays 22-23). Results of
all these assays are not presented in detail
here, but extracts of isolates of all species
tested so far have shown a response in at
least some of these bioassays, as can be
seen in Table 1. These results suggest that
compounds other than MTX-1 and CTXs
may be produced by the species listed in
Table 1. Other toxins known to be pro-
duced by Gambierdiscus species include
gambieric acids, gambieroxide, gambierol
and gambierone 20. Despite the raft of
methods available, the toxicity of isolates
of Gambierdiscus still requires resolution
to improve the knowledge of the function-
al biogeography of the genus.
The risk of ciguatera poisoning is
clearly Pacific-wide and in some islands
has caused many illnesses and even deaths
24. The poisoning can have serious eco-
nomic impacts, particularly on smaller
Pacific Island communities 25. The key
producer of CTXs, G. polynesiensis, has
been found from the far western edges of
the Pacific (Vietnam) to eastern French
Polynesia (Fig. 3) and is likely to be
found further south with warming seas. Of
course, the greater sampling efforts being
undertaken are likely to add to the increas-
ing detections.
Species such as Ostreopsis ovata and
Coolia malayensis occur in the Cook Is-
lands and French Polynesia, where G.
polynesiensis has been reported. These
co-occurring species with Gambierdis-
cus are also found in Melanesia, for ex-
ample, Tongatapu, Kingdom of Tonga
(Phoebe Argyle, unpublished data; Fig.
1) and the Fiji Islands. They also occur
in Micronesia, for example, the Republic
of the Marshall Islands, the US territory
of Guam, and Tol-truk, Federated States
of Micronesia 3. This suggests that the
environmental conditions may be suitable
for the expansion of G. polynesiensis to
these areas.
State-of-the-art molecular tools are
likely to result in increased reports of all
the known species of Gambierdiscus glob-
ally and assist in the discovery of new spe-
cies. DNA sequencing data is critical for
4
descriptions of new species as conserva-
tive morphological differences can be dif-
ficult to identify. Quantitative PCR assays
and metabarcoding are already generating
considerably more data than can be gener-
ated by traditional methods. New species
records are being made at new sites, for
example, in Northland, New Zealand, and
the Cook Islands (K. Smith, unpubl. data).
Ciguatera fish poisoning has been
known in the tropical Pacific region for
centuries, but the increase in species num-
bers and the expansion of the geographic
range of Gambierdiscus species over the
last few years (for example, G. carpenteri
in NSW, Australia 31; Fig. 3) suggests
that CFP will also become of greater con-
cern for more temperate regions in the Pa-
cific in the future.
Acknowledgements
We would like to acknowledge the team
work, in particular with the University
of Technology, Sydney, Australia (led by
Prof Shauna Murray), the Ministry of
Marine Resources, Cook Islands (Dor-
othy Solomona) and the Office of the
Prime Minister, Climate Change Cook
Islands (Dr Teina Rongo). Much of the
Northland and Kermadec Islands, NZ,
research has been funded by the NZ
Ministry for Business, Innovation and
Employment, Contract CAWX1317.
References
1. Parsons M et al 2012. Harmful Algae 14:
107-129
2. Litaker RW et al 2010. Toxicon 56: 711-
730
3. Kohli GS et al 2016. In: Botana LM et al
(eds) Climate change and marine and
freshwater toxins, Anal Bioanal Chem
408, Chapt 9
4. Richlen ML 2008. Harmful Algae 7: 614-
629
5. Guiry MD & Guiry GM 2016. http://www.
algaebase.org; searched on 07 Dec 2016
6. Litaker et al 2009. Phycologia 48: 344-390
7. Fraga S et al 2016. Harmful Algae 58:
93-105
8. Smith KF et al 2016. Harmful Algae 60:
45-56
9. Kretzschmar AL et al 2016. J Phycol
DOI:10.1111/jpy.12496
10. Fraga S & Rodríguez F 2014. Protist 165:
839-853
11 Rhodes LL et al 2016. NZ J Mar Fresh Res,
DOI.org/10.1080/00288330.2016.127
0337
12. Rhodes L et al 2013. NZ J Mar Fresh Res
48: 303-310
13. Murray S et al 2014. Harmful Al-
gae.39:242-252.
14. Gómez et al 2015. PlosOne 10(4):
e0119676. doi:10.1371
15. Chang FH 1996. Shellfish toxin update.
Seafood New Zealand 26, February 1996.
16. Kohli GS et al 2014. Harmful Algae 39:
134-145
17. Vergés A et al 2014. P R Soc B 281: 1-10
18. http://climatechange.environment.nsw.
gov.au/About-climate-change-in-NSW/
Evidence-of-climate-change/Observed-
Australian-climate-change
19. Ministry for the Environment 2016.
Climate Change Projections for New
Zealand. Ministry for the Environment,
127 pp
20. Selwood A et al 2015. In: MacKenzie L
(ed) Marine and freshwater harmful
algae. Proc 16 th Int Conf Harmful Algae,
New Zealand, Oct 2014. (Cawthron Inst
& ISSHA) pp 66-69
21. Lewis RJ 2006. Toxicon 48: 799-809
22. Tester P 2015. In: MacKenzie (ed).
Marine and freshwater harmful algae.
Proc 16 th Int Conf Harmful Algae, New
Zealand, Oct 2014 (Cawthron Inst. & IS-
SHA) pp 44-48
23. Argyle PA et al 2016. NZ J Mar Fresh Res
50: 444-456
24. Rongo T & van Woesik R 2013. Toxicon
64: 8795
25. Rhodes L & R Munday 2016. In: Chisti
Y & F Bux (eds). Algae biotechnology:
products and processes (Springer, UK),
pp 301-316
26. Chinain M et al 1999. J Phycol 35: 1282-
1296
27. Chinain M et al 2010. Toxicon 56: 739-
750
28. Leaw CP et al 2011. Phycol Res 59: 143-
146
29. Tawong W et al 2016. Phycologia 55:
274-278
30. Nishimura T et al 2014. J Phycol 50:
506-514
31. Kohli GS et al 2014. Harmful Algae 39:
134-145
Authors
Lesley Rhodes, Kirsty Smith, Tim Harwood,
Sam Murray, Laura Biessy, Cawthron Insti-
tute, Nelson, New Zealand
Phoebe Argyle, University of Canterbury,
Christchurch, New Zealand
Rex Munday, AgResearch, Hamilton, New
Zealand
Email: Lesley.rhodescawthron.org.nz
HARMFUL ALGAE NEWS NO. 56 / 2017
Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 56 - March 2017 www.ioc-unesco.org/hab Is Gambierdiscus expanding its geographic range in the Pacific region? Content HAB events and toxin effects Gambierdiscus distribution in the Pacific . ...................................
Table 1: Geographic occurrence and toxin production (as determined by LCMS/MS) of Gambierdiscus species in the Pacific region. Species reported in the Pacific region G. australes G. balechii G. belizeanus G. caribaeus G. carpenteri G. cheloniae G. lapillus G. pacificus G. polynesiensis G. scabrosus
Fig. 2. Scanning electron micrographs of cells of Gambierdiscus sp., isolate CAWD242, from the Kermadec Islands: (A) view of cingulum and apical pore, (B) apical pore complex, (C) antapi- cal hypotheca. this will bring the number of Gambier- discus species to fifteen. A related species, originally d
lished data). The role of MTXs in ciguat- era fish poisoning is uncertain and due to its low oral toxicity may only play a small part in toxic events 21. A range of cell-based assays is avail- able to detect CFP-related toxins, includ- ing receptor binding (RBA), Ca 2+ flux, N2A and haemolytic assay
Toxin production in lab-rat-diatoms (e.g. Pseudo-nitzschia) in the presence of copepods From only 15 species in 1965, currently 49 Pseudo-nitzschia species have been described, of which 22 have the abil- ity to produce the toxin domoic acid (DA), along with two Nitzschia species 1. Both numbers are
Apart from increasing toxin produc- tion in already toxic species/strains, presence of grazers may even induce toxin production in species previously considered non-toxic, as shown for P. obtusa 3. It is therefore relevant to consider that all Pseudo-nitzschia spe- cies might have the potential to p
Ecology of Alexandrium spp. in the Strait of Georgia, British Columbia, Canada 2015 Fig. 1 Citizen Science sampling stations in the Strait of Georgia, Canada in 2015. Fig. 2. Citizen Science sampling areas in the Strait of Georgia, Canada in 2015. Coastal waters of British Columbia (BC), Canada have
Table 1. Number of water samples (N) collected by the Citizen Science Program, frequency of samples with Alexandrium spp. presence (Alex. +), and number of water samples with Alexan- drium spp. cells present by month, Strait of Georgia 2015 (n/a - no samples were collected). Area Ladysmith Cowichan
A clarification of three unnamed raphidophytes previously described from British Columbia gated motile cell with very similar chlo- roplasts and the oval Lugol-fixed cell in Fig. 2 #3 were probably equivalent to the long cells formed by V. globosus in stationary growth (see 5 Figs. 7 & 8). Raphidoph
Fig. 3. Raphidophyte 2 from a culture in 1974. Motile cell, ventral view 11.5 μm long. reproduced here at a smaller size (Fig. 4) and are interpreted (in the figure caption) by the author. Photographs of motile cells under stress received from Nicky Haigh (Mi- crothalassia) (not shown here) reveal t
Autumnal algal bloom succession in northern coasts of Gulf of Oman Fig. 1. Map of the study area The composition of harmful algal blooms is determined by the diversity and abundance of different species pre- sent. Temporal fluctuations in phyto- plankton populations can lead to a suc- cession of dif
Table 1. Maximal mean density of the observed bloom species and physical-chemical properties of the near-shore waters during autumnal algal blooms in the northern Gulf of Oman Date 26 October 2016 04 November 2016 09 November 2016 29 November 2016 03 December 2016 Species/ Genus Alexandrium sp. Pror
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The Scottish Coastal Observatory Scotlands coastal environment is sub- ject to inherent variability resulting from short term processes such as tides and weather, the seasonal cycle, multi- annual cycles such as the North Atlantic Oscillation and larger scale influences such as climate change and oc
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IOC/WESTPAC Scientists Shape the Future of Regional HAB Research More than twenty experts from coun- tries in the Western Pacific gathered in Nha Trang, Vietnam, 19-22 December 2016 to foster cooperation and define research priorities for Harmful Algal Blooms (HABs) in the region (Fig. 1). The East
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supply, marine resources, climate change impacts and environmental protection. His voice and perspec- tive will be sorely absent. Paul will be greatly missed by those he touched, but especially by Victoria, his wife of 51 years, and their children Rachel, Richard and Christina. A scholarship fund th
11th International Conference on Modern and Fossil Dinoflagellates The University of Bordeaux (France) is the organiser of the 11 th International Conference on Modern and Fossil Dino- flagellates, DINO11, which will be held from 17 to 21 July 2017 at Bordeaux, France. This series of conference was
IOC Qualifications in Identification and Enumeration of Harmful Marine Microalgae A now traditional IOC course on identi- fication of harmful marine microalgae, including optional workshops on enu- meration and culture techniques. The 2017 course will be held from 6 to 19 August at the IOC Science a
Taxonomic Note Oceanography special issue Alexandrium catenella vs. A. fundyense The Nomenclature Committee for Algae has decided (Prudhomme van Reine, 2017) that the name Gonyaulax catenel- la (Alexandrium catenella) should not be rejected and that A. fundyense and A. catenella are conspecific havi