Who turned on the light? First report of extensive bioluminescent blooms of the heterotrophic dinoflagellate Noctiluca
scintillans with low abundance of bioluminescent bacteria
in the Gulf of Nicoya, Costa Rica
Historically, the Gulf of Nicoya has experienced recurrent algal blooms, including events with the potential to affect
marine organisms and human activities
[1]. This gulf receives freshwater discharges from the Tempisque and Grande
de Tárcoles rivers, together with several smaller tributaries, whose inputs
strongly influence circulation patterns,
salinity gradients, and the transport of
nutrients and organic matter within the
system [2]. These processes, combined
with the gulfs morphology and water
residence time, regulate the mixing
of continental and marine waters and
modulate the distribution and dispersion of phytoplankton and other planktonic organisms, ultimately influencing their composition, abundance, and
temporal dynamics [34]. The coastal
waters surrounding sites as Paquera,
Isla Cedros, Punta Cuchillo, and adjacent areas have exhibited recurrent discoloration events associated with algal
blooms for several decades [1,5].
In February 2026, an extensive
bloom characterized by intense orange
discoloration visible to the naked eye
occurred in the region of the Gulf of Nicoya. The bloom formed with continuous surface aggregations with an oily
appearance and texture, likely associated with exceptionally high cell densities
observed during the event. These patches were distributed across the water
surface and were transported by local
currents (Fig. 1). At night-time, strong
and intense blue bioluminescence was
observed in the Paquera area following mechanical disturbance caused by
waves and boat movement (Fig. 2).
During this event, surface water
samples were collected both day and
night from several locations near Cedros Island (Fig. 1). The samples were
analyzed using optical microscopy to
identify the bloom-forming microalgae
and associated phytoplankton species.
In addition, the abundance of total cultivable heterotrophic and bioluminescent
bacteria (CFU mL1) was determined
by filtering 5, 1, and 0.1 mL aliquots
through 0.22 μm cellulose nitrate membrane filters. These filters were subsequently inoculated onto LSW-70 agar
and incubated at 28 C for 24 h (Fig. 2).
Water temperature during the event
ranged from 27 to 28 C, with a salinity
of 32. According to the National Meteorological Institute of Costa Rica (IMN),
January and February were characterized by warm and predominantly dry
conditions typical of the North Pacific
dry season. Persistent northeasterly
winds were recorded, with moderate
to strong intensity (3040 km h-1), to-
Fig. 1. Extensive orange-colored patches produced by the dinoflagellate Noctiluca scintillans
around Cedros Island and the Gulf of Nicoya. (A) Map of the Gulf of Nicoya (94909 N and
845556 W). (B, C) Extensive patches with orange coloration (photographs by Marisol Hidalgo
Prado).
HARMFUL ALGAE NEWS NO. 83 / 2026
gether with mostly clear skies. Several
cold front events occurred in February,
which further enhanced wind activity in
the region.
Sample analyses revealed that the
bloom was produced almost exclusively by the heterotrophic dinoflagellate
Noctiluca scintillans, whose size ranged
from 180 to 250 μm (Fig. 2). Minor
amounts of the dinoflagellate Prorocentrum micans and the central diatom
Stephanocyclus meneghinianus (=Cyclotella meneghiniana) were observed.
As the water samples were highly concentrated with N. scintillans, accurate
cell counting was difficult; therefore,
several counts were performed using
Utermöhl chambers, yielding concentrations of 1.7 103 to 1 106 cells L1.
Noctiluca scintillans is a heterotrophic dinoflagellate that feeds on
diatoms and bacteria, among other organisms. However, both in the water
samples and inside Noctiluca cells, a
high abundance of the planktonic ciliate
Strombidium sp. was observed. This oligotrich ciliate is approximately 20 μm
in diameter, and functions as a microzooplankton grazer feeding on bacteria
and pico- to nanophytoplankton, and is
commonly abundant in coastal and oceanic waters (Fig. 2). Although not previously reported in the Gulf of Nicoya, it
is a widespread component of marine
microzooplankton and plays a key role
in microbial food webs. Strombidium
spp. consume small dinoflagellates and
diatoms and can exert grazing pressure
on phytoplankton blooms. However,
this effect is limited when blooms are
dominated by larger dinoflagellates
such as N. scintillans, which is itself a
predator of smaller plankton. Consequently, in systems such as the Gulf of
Nicoya, Strombidium may contribute to
the regulation of small phytoplankton,
but its effect diminishes under blooms
dominated by N. scintillans.
Strombidium is associated with relatively oligotrophic environments, thriving in nutrient-poor tropical or subtropical waters. It is widely distributed
in tropical, subtropical, and temperate
17
Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 83 June 2026 https://hab.ioc-unesco.org/ Long and Winding Sea-lanes for Fish-Killing Algal Events An ancient idiom dead fish rot (or stink) from the head down possibly attributable to Turkish or Persian fishers but the orig
Fig. 2. Programme for the Advanced International Colloquium and Technical Workshop on fish killing marine algae and their effects. blooms. The WG also decided to revise the classic but outdated Cooperative Research Report [2] on HAB effects on mariculture and marine fisheries published in 1992 for
Chilean government, through CORFO and cooperation of CREAN-IFOP (reported in HAN 63 [3]) (Fig. 2). The colloquium convenors invited international experts to Puerto Varas, Chile in 2019 to review disciplinary knowledge on all aspects of fish-killing algae and associated mortality events (Fig. 3). A p
ins were added during the IPHAB XVII Intersessional (2025-2026), but the total meagre reported ichthyotoxins score (by March 2025) (zero goniodomins, zero prymnesins, one karlotoxin [sterolysin]) has increased dramatically (by February 2026): seven goniodomins, four prymnesins, one karlotoxin, and m
(admittedly controversial) explain how toxigenic blooms may directly kill fish in aquaculture operations [e.g., 11]. Access to comprehensive time-series databases on HAB events (HAEDAT, HAIS/ OBIS) has allowed for interpretation of fish-killing events over decades on a regional geographical basis, e
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Who turned on the light? First report of extensive bioluminescent blooms of the heterotrophic dinoflagellate Noctiluca scintillans with low abundance of bioluminescent bacteria in the Gulf of Nicoya, Costa Rica Historically, the Gulf of Nicoya has experienced recurrent algal blooms, including events
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