Fig. 3. Light microscope images of living Fibrocapsa japonica cells.
Scale bars = 20 μm.
French waters. REPHY data [9] indicate
that the previous maximum abundance
occurred in 2013 in the Vilaine estuary,
reaching 1.9 x 105 cells L1, approximately 25 times lower than values than
maximum concentrations observed in
2025.
Although associated with fish mortality events in Japan in the 1970s [7],
no mortality events were reported
during this bloom period in France.
Some fishermen nevertheless reported
unusually low fish abundance in Concarneau Bay and the Morbihan Gulf. An
important mortality event involving Atlantic puffins (Fratercula arctica) was
also recorded in southern Brittany in
the weeks following the bloom (February). While the successive storms that
hit the region during this period appear
to have been the primary cause of mortality, a reduction in fish availability
the puffins main prey linked to the
bloom may have further weakened the
birds, making them more vulnerable.
Fig. 5. Viability percentage (expressed relative
to solvent control L1 medium at 25%) of fish
gill cells (RTgill-W1 cell line), after two-hour
exposure to different concentrations of crude
intracellular fractions from the French F.
japonica strain IFR-CC 25-055. Different letters
indicate significant differences (ANOVA followed by Tukey HSD post-hoc test, p<0.05, n = 6
replicate wells).
HARMFUL ALGAE NEWS NO. 83 / 2026
Fig. 4. Temporal evolution of F. japonica abundances (cells L-1) recorded at
REPHY stations during the bloom (November 2025 to February 2026).
According to the Ligue pour la Protection des Oiseaux (LPO), 45,014 puffins
were found beached along the French
Atlantic coast between 19 December
2025 and 17 March 2026. This figure
represents only a fraction of the total
mortality, as experts estimate that only
about one in ten birds reaches the shore
[10]. In vitro bioassays were performed
to assess the ichthyotoxic potential of
a French strain (F. japonica IFR-CC 25055), isolated from a single-cell from the
bloom. Intracellular content, obtained
from the supernatant of lysed culture
pellets, were tested on the RTgill-W1
fish gill cell line (CRL-2523, ATCC), following Beesoo et al. [11] and Rolton et
al. [12]. No significant cytotoxicity was
observed between 4.0 x 105 to 6.2 x 106
cells L1 (Fig. 5). A significant reduction
in cell viability occurred only above 2.5
x 107 cells L1 (Fig. 5). The maximum
environmental concentration observed
(4.8 106 cells L1) therefore suggests limited cytotoxic potential under
natural conditions. By contrast, Aguiar
Juárez et al. [13] reported cytotoxicity
on RTgill-W1 for Argentinian strains of
F. japonica at much lower cell densities,
(6.2 x 105 cells L1), highlighting straindependent toxicity modulated by environmental and physiological factors
such as salinity, temperature, light intensity and nutrient availability. While
the French strain appears weakly toxic
under tested conditions, further comparative studies are needed to assess
variability among strains and growth
conditions.
This event highlights the value of
integrating fixed-station phytoplankton monitoring with citizen science observations for early detection of bloom
events. It also demonstrates the importance of combining taxonomy, molecular biology, hydrology, chemistry and
ecotoxicology for comprehensive characterisation of harmful algal events.
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Authors
Ewenn Cochet, Audrey Duval, Amélie Derrien,
Malwenn Lassudrie, Nicolas Chomérat, &
Gwenael Bilien, Ifremer, COAST, F-29900
Concarneau, France
Michael Retho, Ifremer, COAST, F-56100
Lorient, France
Email corresponding author:
ewenn.cochet@ifremer.fr
https://doi.org/10.5281/zenodo.20583225
9
Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 83 June 2026 https://hab.ioc-unesco.org/ Long and Winding Sea-lanes for Fish-Killing Algal Events An ancient idiom dead fish rot (or stink) from the head down possibly attributable to Turkish or Persian fishers but the orig
Fig. 2. Programme for the Advanced International Colloquium and Technical Workshop on fish killing marine algae and their effects. blooms. The WG also decided to revise the classic but outdated Cooperative Research Report [2] on HAB effects on mariculture and marine fisheries published in 1992 for
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