from which the species name cristata is
derived), and a longer hypocone with
the right lobe slightly longer than the
left. Scanning electron microscopy revealed that the crest was formed by a
slight elevation of the right side of the
apical groove. On the dorsal side, the
apical groove extended to one-third of
the epicone length. Living cells exhibited a large centrally located nucleus
(N), unlike K. mikimotoi, in which the
nucleus is positioned on the left side
of the hypocone. Upon Lugols iodine
preservation, cells often displayed a
more pointed epicone and a more pronounced apical crest, with the nucleus
positioned lower in the hypocone. The
sulcal intrusion (si) was either wideopen or terminated in a finger-like projection. This species closely resembles
the Chinese Karenia hui (also possessing an apical crest), as well as Karenia
selliformis (but with two equal hypoconal lobes). Both latter species differ in
possessing a large elongate hypoconal
nucleus. A qPCR assay for K. hui and K.
selliformis showed no cross-reactivity
with K. cristata (from cultures or environmental samples), while K. brevis was
never detected in the South Australian
bloom [5].
The environmental drivers behind
this unprecedented HAB are currently
subject of intense investigation. A sustained massive upwelling event started
in the summer of 2023/2024 (Fig. 1).
Other notable oceanographic events
over the period were a marine heat
wave in early 2025, characterised by
water temperatures approximately 2
degrees warmer than normal in the
months preceding the HAB, and nutrient enrichment several years earlier
associated with flooding of the Murray/Darling river system. The Great
Southern Australian Upwelling System
[9] commonly triggers diatom blooms
that support high productivity of sardine and tuna populations, as well as a
high biodiversity of seals, whales, dolphins, sharks, and seabirds. In the past,
upwelling relaxation in this region has
only rarely led to inshore Karenia mikimotoi dinoflagellate blooms (e.g. 2014
in Coffin Bay). The multifactorial conditions that facilitated Karenia cristata to
newly generate such long-lasting, persistent ecosystem disruptive HAB event
inside the Spencer Gulf and Gulf of St
Vincent remain unresolved.
HARMFUL ALGAE NEWS NO. 83 / 2026
Fig. 3. Karenia cristata from South Australia. Light micrographs of (A) living and (B) Lugol-preserved cells, showing the apical crest (cr) and nucleus position (N). Scanning electron micrographs
of (C) a whole cell in ventral view, showing the right hypothecal lobe longer than the left lobe; (D)
high-magnification detail of the ventral view of the sulcal intrusion (si), with the right edge (arrow)
of the straight apical groove (ag) elevated; and (E) dorsal view of the apical groove extending onethird of the length of the epitheca. Images: (A) S. Murray; (B) C. Wilkinson; (CE) G. Hallegraeff.
This recent South Australian HAB
has highlighted a critical need to
strengthen preparedness frameworks
at Australian state and national levels.
Long-term HAB management is currently not specifically addressed in existing environmental, biosecurity, human health or climate-change policies
in Australia [10]. A professional network for researchers and stakeholders
in marine HAB science was established
in 2025 with the formation of ANZHABNET (11), and funding for HAB monitoring, mitigation, restoration, and research has begun to be made available.
The implications of this event for the
global HAB community are profound.
We now recognize a second high-brevetoxin producing Karenia species that
poses a new threat to marine ecosystems and human health. This species
distribution and ecology needs to be
urgently investigated, not just in Australia, but any waters with comparable
conditions.
References
1. Hallegraeff G 2024. Harmful Australian
Marine Microalgae. CSIRO Publishing
2. Murray SA & Gaiani G 2025. The Conversation, 24 March 2025.
3. PIRSA 2014. Fisheries and Aquaculture
Division Report Adelaide: Primary Industries and Regions South Australia 31.
4. Verma A et al. 2024. Frontiers in
Protistology 2, p.1422481. https://doi.
org/10.3389/frpro.2024.1422481
5. Murray SA et al. 2025. bioRxiv. https://
doi.org/10.1101/2025.10.31.685766
6. Botes L et al. 2003. Phycologia 42,
563571. https://doi.org/10.2216/
i0031-8884-42-6-563.1
7. Lassus P et al. 2016. Toxic and harmful microalgae of the world ocean. Cont
Shelf Res. Intergovernmental Oceanographic Commission of UNESCO. IOC
Manuals and Guides 68, plate 32 fig B.
https://unesdoc.unesco.org/ark:/48223/
pf0000247767
8. Orlova TY et al. 2022. Harmful Algae
120, 102337. https://doi.org/10.1016/j.
hal.2022.102337
9. Kämpf J 2026. Continental Shelf Research
297, 105631. https://doi.org/10.1016/j.
csr.2025.105631
10. Baum F et al 2026. Health Promotion International 41. https://doi.org/10.1093/
heapro/daaf240
11. Turnbull et al. 2026. Formation of ANZ
HABNET in response to an unprecedented HAB in South Australia, 20252026.
In Reguera B & Mertens KN (Eds) HAN
82, UNESCO. pp. 611. https://doi.
org/10.5281/zenodo.19045619
Authors
Gustaaf Hallegraeff, Christopher Bolch
& Alison Turnbull, University of Tasmania
Steve Brett, Microalgal Services
Hazel Farrell, NSW Department of Primary
Industries and Regional Development,
Ruth Eriksen, CSIRO Australia
Tim Harwood & Kirsty Smith, Cawthron
Institute, New Zealand
Greta Gaiani & Shauna Murray, University
of Technology Sydney
Email corresponding author:
Shauna.Murray@uts.edu.au
https://doi.org/10.5281/zenodo.20583164
7
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