from which the species name cristata is derived), and a longer hypocone with the right lobe slightly longer than the left. Scanning electron microscopy revealed that the crest was formed by a slight elevation of the right side of the apical groove. On the dorsal side, the apical groove extended to one-third of the epicone length. Living cells exhibited a large centrally located nucleus (N), unlike K. mikimotoi, in which the nucleus is positioned on the left side of the hypocone. Upon Lugols iodine preservation, cells often displayed a more pointed epicone and a more pronounced apical crest, with the nucleus positioned lower in the hypocone. The sulcal intrusion (si) was either wideopen or terminated in a finger-like projection. This species closely resembles the Chinese Karenia hui (also possessing an apical crest), as well as Karenia selliformis (but with two equal hypoconal lobes). Both latter species differ in possessing a large elongate hypoconal nucleus. A qPCR assay for K. hui and K. selliformis showed no cross-reactivity with K. cristata (from cultures or environmental samples), while K. brevis was never detected in the South Australian bloom [5]. The environmental drivers behind this unprecedented HAB are currently subject of intense investigation. A sustained massive upwelling event started in the summer of 2023/2024 (Fig. 1). Other notable oceanographic events over the period were a marine heat wave in early 2025, characterised by water temperatures approximately 2 degrees warmer than normal in the months preceding the HAB, and nutrient enrichment several years earlier associated with flooding of the Murray/Darling river system. The Great Southern Australian Upwelling System [9] commonly triggers diatom blooms that support high productivity of sardine and tuna populations, as well as a high biodiversity of seals, whales, dolphins, sharks, and seabirds. In the past, upwelling relaxation in this region has only rarely led to inshore Karenia mikimotoi dinoflagellate blooms (e.g. 2014 in Coffin Bay). The multifactorial conditions that facilitated Karenia cristata to newly generate such long-lasting, persistent ecosystem disruptive HAB event inside the Spencer Gulf and Gulf of St Vincent remain unresolved. HARMFUL ALGAE NEWS NO. 83 / 2026 Fig. 3. Karenia cristata from South Australia. Light micrographs of (A) living and (B) Lugol-preserved cells, showing the apical crest (cr) and nucleus position (N). Scanning electron micrographs of (C) a whole cell in ventral view, showing the right hypothecal lobe longer than the left lobe; (D) high-magnification detail of the ventral view of the sulcal intrusion (si), with the right edge (arrow) of the straight apical groove (ag) elevated; and (E) dorsal view of the apical groove extending onethird of the length of the epitheca. Images: (A) S. Murray; (B) C. Wilkinson; (CE) G. Hallegraeff. This recent South Australian HAB has highlighted a critical need to strengthen preparedness frameworks at Australian state and national levels. Long-term HAB management is currently not specifically addressed in existing environmental, biosecurity, human health or climate-change policies in Australia [10]. A professional network for researchers and stakeholders in marine HAB science was established in 2025 with the formation of ANZHABNET (11), and funding for HAB monitoring, mitigation, restoration, and research has begun to be made available. The implications of this event for the global HAB community are profound. We now recognize a second high-brevetoxin producing Karenia species that poses a new threat to marine ecosystems and human health. This species distribution and ecology needs to be urgently investigated, not just in Australia, but any waters with comparable conditions. References 1. Hallegraeff G 2024. Harmful Australian Marine Microalgae. CSIRO Publishing 2. Murray SA & Gaiani G 2025. The Conversation, 24 March 2025. 3. PIRSA 2014. Fisheries and Aquaculture Division Report Adelaide: Primary Industries and Regions South Australia 31. 4. Verma A et al. 2024. Frontiers in Protistology 2, p.1422481. https://doi. org/10.3389/frpro.2024.1422481 5. Murray SA et al. 2025. bioRxiv. https:// doi.org/10.1101/2025.10.31.685766 6. Botes L et al. 2003. Phycologia 42, 563571. https://doi.org/10.2216/ i0031-8884-42-6-563.1 7. Lassus P et al. 2016. Toxic and harmful microalgae of the world ocean. Cont Shelf Res. Intergovernmental Oceanographic Commission of UNESCO. IOC Manuals and Guides 68, plate 32 fig B. https://unesdoc.unesco.org/ark:/48223/ pf0000247767 8. Orlova TY et al. 2022. Harmful Algae 120, 102337. https://doi.org/10.1016/j. hal.2022.102337 9. Kämpf J 2026. Continental Shelf Research 297, 105631. https://doi.org/10.1016/j. csr.2025.105631 10. Baum F et al 2026. Health Promotion International 41. https://doi.org/10.1093/ heapro/daaf240 11. Turnbull et al. 2026. Formation of ANZ HABNET in response to an unprecedented HAB in South Australia, 20252026. In Reguera B & Mertens KN (Eds) HAN 82, UNESCO. pp. 611. https://doi. org/10.5281/zenodo.19045619 Authors Gustaaf Hallegraeff, Christopher Bolch & Alison Turnbull, University of Tasmania Steve Brett, Microalgal Services Hazel Farrell, NSW Department of Primary Industries and Regional Development, Ruth Eriksen, CSIRO Australia Tim Harwood & Kirsty Smith, Cawthron Institute, New Zealand Greta Gaiani & Shauna Murray, University of Technology Sydney Email corresponding author: Shauna.Murray@uts.edu.au https://doi.org/10.5281/zenodo.20583164 7 Harmful Algae News An IOC Newsletter on Toxic Algae and Algal Blooms No. 83 June 2026 https://hab.ioc-unesco.org/ Long and Winding Sea-lanes for Fish-Killing Algal Events An ancient idiom dead fish rot (or stink) from the head down possibly attributable to Turkish or Persian fishers but the orig Fig. 2. Programme for the Advanced International Colloquium and Technical Workshop on fish killing marine algae and their effects. blooms. The WG also decided to revise the classic but outdated Cooperative Research Report [2] on HAB effects on mariculture and marine fisheries published in 1992 for Chilean government, through CORFO and cooperation of CREAN-IFOP (reported in HAN 63 [3]) (Fig. 2). The colloquium convenors invited international experts to Puerto Varas, Chile in 2019 to review disciplinary knowledge on all aspects of fish-killing algae and associated mortality events (Fig. 3). A p ins were added during the IPHAB XVII Intersessional (2025-2026), but the total meagre reported ichthyotoxins score (by March 2025) (zero goniodomins, zero prymnesins, one karlotoxin [sterolysin]) has increased dramatically (by February 2026): seven goniodomins, four prymnesins, one karlotoxin, and m (admittedly controversial) explain how toxigenic blooms may directly kill fish in aquaculture operations [e.g., 11]. Access to comprehensive time-series databases on HAB events (HAEDAT, HAIS/ OBIS) has allowed for interpretation of fish-killing events over decades on a regional geographical basis, e Catastrophic marine mass mortalities, shellfish toxicity and human respiratory problems from a Karenia cristata dinoflagellate bloom in South Australia, 20252026 Fig. 1. Satellite chlorophyll image from March 2024 showing widespread offshore diatom blooms in response to a massive upwelling event du from which the species name cristata is derived), and a longer hypocone with the right lobe slightly longer than the left. Scanning electron microscopy revealed that the crest was formed by a slight elevation of the right side of the apical groove. On the dorsal side, the apical groove extended to o Unprecedented bloom of Fibrocapsa japonica on French coasts Fig. 1. Map of the different bays in southern Brittany monitored as part of the REPHY program. Since 1987, the REPHY (French Phytoplankton and Hydrology Monitoring Network in Coastal Waters), operated by IFREMER, has conducted long-term m Fig. 3. Light microscope images of living Fibrocapsa japonica cells. Scale bars = 20 μm. French waters. REPHY data [9] indicate that the previous maximum abundance occurred in 2013 in the Vilaine estuary, reaching 1.9 x 105 cells L1, approximately 25 times lower than values than maximum concentrati First Record of Fukuyoa sp. (Gambierdiscoideae) in the Northeastern Region of Términos Lagoon, Campeche, Mexico Fig. 1. Map of sampling stations at Términos Lagoon, Campeche, Mexico. The station where Fukuyoa sp. was found is circled in red. Términos Lagoon is a region of substantial economic rele Fig. 2. (AB). Fukuyoa sp. in ventral view. (C). Dorsal view. (D). Antapical view showing plates S. d. p., 1, 1. (EF). Ventral view showing plates 1, 1,2, 7, S. d. p., 1,1. (GH). Antapical view showing plates 1p,2,3, 1, 2. Scale bars = 20 μm. in Australia [6] recorded F. paulensis at temperatures of Trophic interactions shape ciguatera risk in a warming ocean Ciguatera is one of the most widespread marine poisonings worldwide, caused by the consumption of fish that bioaccumulate ciguatoxins (CTXs) produced by dinoflagellate species belonging to the genus Gambierdiscus. Its expansion into non-en drivers such as temperature variability and habitat disturbance. Calibration and validation with empirical data on cell densities, toxin concentrations, and ecosystem dynamics will be essential to generate robust predictions. The inclusion of spatial structure and coupling with human health risk mod Red and green waters in southern B rittany (France) in March 2026 linked to a bloom of Mesodinium spp. Fig. 1. Map of reported water discoloration events in southern Brittany (1012 March 2026) based on PHENOMER observations and direct reports. Locations include the Bay of Audierne and the Bay of C Fig. 3. Sentinel-2 satellite image (11 March 2026) showing the spatial extent of the bloom in southern Finistère, with burgundy-red patches indicative of high Mesodinium surface concentrations. are more stable and impart a green coloration. This transformation can occur within minutes, explaining t Fig. 6. Cells of Mesodinium major. 13. Different views of living cells. 46. Representation of pigment dynamics during Mesodinium cell degradation: transition from phycoerythrin-dominated red coloration to chlorophyll-dominated green coloration following cell lysis. All images to scale. accompanied Who turned on the light? First report of extensive bioluminescent blooms of the heterotrophic dinoflagellate Noctiluca scintillans with low abundance of bioluminescent bacteria in the Gulf of Nicoya, Costa Rica Historically, the Gulf of Nicoya has experienced recurrent algal blooms, including events Fig. 2. Dominant dinoflagellate of the Noctiluca scintillans algal bloom around Cedros Island. (A) Bloom of N. scintillans. (B) Ciliate fed on by Noctiluca, Strombidium sp. (C) Culture of bioluminescent bacteria. (D, E) bioluminescence observed at night on Cedros Island and surrounding areas (photog estuaries supporting fisheries, tourism, and biodiversity. Monitoring these processes allows differentiation between benign and harmful blooms and supports the preservation of ecosystem integrity. Ultimately, such phenomena not only expand scientific understanding but also underscore the resilience International Conference on Molluscan Shellfish Safety (ICMSS) Dear colleagues, We wanted to draw your attention to the upcoming International Conference on Molluscan Shellfish Safety (ICMSS), taking place 611 September 2026 at the University of Exeter, UK. Further details, including registration (w DART in action: Scientists launch regional effort against toxic diatoms Scientists from across Asia have come together to tackle the growing threat of toxic diatoms that produce neurotoxin Domoic Acid (DA). On 1718 March 2026, researchers from China, Malaysia, and Singapore gathered in Qingdao, Chin Fig. 2. Kick-off meeting and research presentations. (A) Nancheng Chen, the lead PI, delivers the opening address. (BD) PIs from participating countries presenting their national research plans. (EF) Selected presentations delivered during the workshop. molecular tools can greatly improve our abilit IOC-FAO Intergovernmental Panel on Harmful Algal Blooms (IPHAB) Extraordinary online Session 27 October 2026 and 18th Session (IPHAB-XVIII), 1820 March 2027, FAO, Rome The Intergovernmental Panel on Harmful Algal Blooms (IPHAB) was established in 1992 to strengthen the scientific, managerial, and fi